Grabowska Iwona, Sharma Neha, Vasilescu Alina, Iancu Madalina, Badea Gabriela, Boukherroub Rabah, Ogale Satishchandra, Szunerits Sabine
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland.
Indian Institute of Science Education and Research (IISER), 411008, Pune, India.
ACS Omega. 2018 Sep 30;3(9):12010-12018. doi: 10.1021/acsomega.8b01558. Epub 2018 Sep 26.
Rapid and accurate diagnostic technologies for early-state identification of cardiovascular abnormalities have become of high importance to prevent and attenuate their progression. The capability of biosensors to determine an increase in the concentration of cardiovascular protein biomarkers in circulating blood immediately after a myocardial infarction makes them ideal point-of-care platforms and alternative approaches to electrocardiograms, chest X-rays, and different laboratory-based immunoassays. We report here a generic approach toward multianalyte sensing platforms for cardiac biomarkers by developing aptamer-based electrochemical sensors for brain natriuretic peptide (BNP-32) and cardiac troponin I (cTnI). For this, commercial gold-based screen-printed electrodes were modified electrophoretically with polyethyleneimine/reduced graphene oxide films. Covalent grafting of propargylacetic acid integrates propargyl groups onto the electrode to which azide-terminated aptamers can be immobilized using Cu(I)-based "click" chemistry. To ensure low biofouling and high specificity, cardiac sensors were modified with pyrene anchors carrying poly(ethylene glycol) units. In the case of BNP-32, the sensor developed has a linear response from 1 pg mL to 1 μg mL in serum; for cTnI, linearity is observed from 1 pg mL to 10 ng mL as demanded for early-stage diagnosis of heart failure. These electrochemical aptasensors represent a step further toward multianalyte sensing of cardiac biomarkers.
用于早期识别心血管异常的快速准确诊断技术对于预防和减缓其进展至关重要。生物传感器能够在心肌梗死后立即测定循环血液中心血管蛋白质生物标志物浓度的增加，这使其成为理想的即时检测平台，也是心电图、胸部X光和不同的基于实验室的免疫测定的替代方法。我们在此报告一种通过开发基于适体的脑钠肽（BNP-32）和心肌肌钙蛋白I（cTnI）电化学传感器来构建用于心脏生物标志物的多分析物传感平台的通用方法。为此，使用聚乙烯亚胺/还原氧化石墨烯薄膜对商业金基丝网印刷电极进行电泳修饰。丙炔酸的共价接枝将炔丙基整合到电极上，使用基于Cu(I)的“点击”化学可以将叠氮端基适体固定在该电极上。为确保低生物污染和高特异性，心脏传感器用带有聚乙二醇单元的芘锚进行修饰。对于BNP-32，所开发的传感器在血清中的线性响应范围为1 pg/mL至1 μg/mL；对于cTnI，根据心力衰竭早期诊断的要求，线性范围为1 pg/mL至10 ng/mL。这些电化学适体传感器代表了朝着心脏生物标志物多分析物传感迈出的又一步。
