Klavinskis L S, Tishon A, Oldstone M B
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
J Immunol. 1989 Sep 15;143(6):2013-6.
The efficiency of cloned class I MHC restricted CTL specific for the nucleoprotein or glycoprotein of lymphocytic choriomeningitis virus in either mediating virus clearance or immunopathologic disease in mice during acute infection was quantitated. Cloned CTL specific for either an internal (nucleoprotein) or surface (glycoprotein) protein of lymphocytic choriomeningitis virus, when administered intracerebrally 5 days after the initiation of infection induced fatal immunopathology, indicating that both internal and surface viral Ag play a role in immune mediated disease in vivo. Dose-response analysis indicated that only 10(2) to 10(3) cloned CTL injected intracerebrally were required to induce mortality in 50% of inoculated syngeneic mice. Thus relatively few virus-specific CTL are required to induce an acute immunopathologic disease in the central nervous system. In contrast, if cloned CTL are adoptively transferred at the time of initiation of viral infection they provide protection as demonstrated by their ability to eliminate virus from the brain and thus terminate the acute infection.
对克隆的、针对淋巴细胞性脉络丛脑膜炎病毒核蛋白或糖蛋白的I类主要组织相容性复合体（MHC）限制性细胞毒性T淋巴细胞（CTL）在急性感染期间介导小鼠病毒清除或免疫病理疾病的效率进行了定量分析。当在感染开始后5天脑内注射时，针对淋巴细胞性脉络丛脑膜炎病毒内部（核蛋白）或表面（糖蛋白）蛋白的克隆CTL诱导了致命的免疫病理学变化，这表明病毒内部和表面抗原在体内免疫介导的疾病中均起作用。剂量反应分析表明，脑内注射仅10²至10³个克隆CTL就能使50%的同基因接种小鼠死亡。因此，诱导中枢神经系统急性免疫病理疾病所需的病毒特异性CTL相对较少。相反，如果在病毒感染开始时过继转移克隆CTL，它们能提供保护，表现为有能力从脑中清除病毒从而终止急性感染。
