Tomioka H, Yamada Y, Saito H, Jidoi J
Department of Microbiology and Immunology, Shimane Medical University, Izumo, Japan.
Int J Lepr Other Mycobact Dis. 1989 Sep;57(3):628-32.
The susceptibilities of Mycobacterium leprae and M. avium complex (MAC) to the H2-O2-Fe-mediated halogenation system supplemented with antimicrobial agents were evaluated by fluorescein diacetate-ethidium bromide (FDA/EB) staining. In the case of M. leprae, the number of greenstained bacteria (intact cells) was reduced in the presence of the H2O2-Fe-mediated halogenation system supplemented with agents possessing antileprosy activity, such as rifampin, 4,4'-diaminodiphenylsulfone (dapsone), clofazimine, and ofloxacin. In the case of the MAC strain, although viable units of the organisms were reduced by the halogenation system alone, the number of greenstained cells in the FDA/EB stain was not reduced, even when the halogenation system was used in combination with ofloxacin. Because stainability of the cells is related to structural and functional intactness of the membrane, differences between M. leprae and the MAC strain imply possible differences in the rigidity of the cell membrane.
通过荧光素二乙酸酯-溴化乙锭（FDA/EB）染色评估了麻风分枝杆菌和鸟分枝杆菌复合群（MAC）对添加抗菌剂的H2-O2-Fe介导卤化系统的敏感性。对于麻风分枝杆菌，在添加具有抗麻风活性的药物（如利福平、4,4'-二氨基二苯砜（氨苯砜）、氯法齐明和氧氟沙星）的H2O2-Fe介导卤化系统存在的情况下，绿色染色细菌（完整细胞）的数量减少。对于MAC菌株，尽管仅卤化系统就使生物体的存活单位减少，但即使卤化系统与氧氟沙星联合使用，FDA/EB染色中绿色染色细胞的数量也没有减少。由于细胞的可染色性与膜的结构和功能完整性有关，麻风分枝杆菌和MAC菌株之间的差异意味着细胞膜刚性可能存在差异。
