Institute of Plant and Environmental Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.
J Insect Sci. 2013;13:135. doi: 10.1673/031.013.13501.
The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is one of the most important pests that has developed high pesticide resistance. The resistances of five Chinese populations of this moth, four resistant strains (from Beijing, Henan, Fujian, and Guangdong) and one susceptible strain, to five pesticides were determined, and the activities of carboxylesterase, glutathione S-transferase, and acetylcholine esterase were tested in all five populations. The correlations between pesticide resistance and enzyme activity were analyzed. The results showed that the resistance status to the five pesticides was different among the five populations. The resistance ratios of the Beijing and Henan populations to spinosad were 5.84 and 8.22, respectively, and those to beta-cypermethrin were 4.91 and 4.98, respectively. These ratios were higher than those for the Fujian and Guangdong populations. The Fujian population was more sensitive to abamectin and chlorpyrifos than the susceptible population (the resistance ratios were 0.14 and 0.91, respectively); in fact, the median lethal concentration for P. xylostella was significantly higher for chlorpyrifos than that for any of the other four pesticides. The carboxylesterase activity in P. xylostella showed positive correlations with the resistance to spinosad, beta-cypermethrin, chlorpyrifos, and abamectin, but no correlation was observed between the carboxylesterase activity and resistance to emamectin benzoate, between glutathione S-transferase activity and resistance to any of the five pesticides tested, or between acetylcholine esterase activity and any of the pesticides except for emamectin benzoate.
小菜蛾，Plutella xylostella（L.）（鳞翅目：菜蛾科），是一种重要的害虫，已发展出高度的抗药性。测定了该蛾的五个中国种群（来自北京、河南、福建和广东的四个抗性种群和一个敏感种群）对五种农药的抗药性，并测试了所有五个种群的羧酸酯酶、谷胱甘肽 S-转移酶和乙酰胆碱酯酶的活性。分析了农药抗性与酶活性之间的相关性。结果表明，五个种群对五种农药的抗性状况不同。北京和河南种群对多杀菌素的抗性比分别为 5.84 和 8.22，对β-氯氰菊酯的抗性比分别为 4.91 和 4.98，均高于福建和广东种群。福建种群对阿维菌素和毒死蜱的敏感性高于敏感种群（抗性比分别为 0.14 和 0.91）；事实上，小菜蛾对毒死蜱的半数致死浓度明显高于其他四种农药。小菜蛾的羧酸酯酶活性与对多杀菌素、β-氯氰菊酯、毒死蜱和阿维菌素的抗性呈正相关，但与对甲氨基阿维菌素苯甲酸盐的抗性无相关性，谷胱甘肽 S-转移酶活性与五种测试农药中的任何一种的抗性均无相关性，乙酰胆碱酯酶活性与除甲氨基阿维菌素苯甲酸盐以外的任何一种农药均无相关性。
