El-Saleh Marwa A, Aioub Ali A, El-Sheikh El-Sayed A, Desuky Wahied M H, Alkeridis Lamya Ahmed, Al-Shuraym Laila A, Farag Marwa M A, Sayed Samy, Aioub Ahmed A A, Hamed Ibrahim A
Plant Protection Research Institute, Agricultural Research Center, Dokki 12618, Giza, Egypt.
Department of Plant Protection, Faculty of Agriculture, Zagazig University, Zagazig 44511, Sharkia, Egypt.
Insects. 2025 Aug 7;16(8):821. doi: 10.3390/insects16080821.
(Boisd.) (Lepidoptera: Noctuidae) is a major insect pest that severely affects various crops. Our study provides new insights by combining field efficacy trials with enzymatic analysis to evaluate the effects of emamectin benzoate mixtures with other insecticides (lufenuron, cypermethrin, chlorpyrifos, and spinosad) against . The aim of our work was to investigate the effectiveness of five insecticides, i.e., emamectin benzoate, lufenuron, cypermethrin, chlorpyrifos, and spinosad, for controlling this pest under field conditions during two consecutive seasons (2023-2024). Each insecticide was applied individually at the recommended rate, while some were mixed with emamectin benzoate at half its recommended rate. The results indicated that emamectin benzoate was the most effective insecticide, followed by lufenuron. The joint action of emamectin benzoate (LC) and its mixtures with other insecticides (chlorpyrifos, spinosad, cypermethrin, and lufenuron) at various concentrations (LC) against second- and fourth-instar larvae was evaluated. Results showed additive effects with chlorpyrifos, lufenuron, and cypermethrin, while potentiation occurred with cypermethrin (LC) and chlorpyrifos (LC). Antagonistic effects were observed in the combination of emamectin benzoate with spinosad (LC and LC). This study concluded that applying insecticides individually is more cost-effective for managing cotton leafworm infestations in cotton crops. Additionally, enzyme activity analysis showed significant changes in alpha-esterase, beta-esterase, carboxylesterase, acetylcholinesterase, and glutathione S-transferase levels in larvae treated with different insecticide combinations.
（博伊斯德）（鳞翅目：夜蛾科）是一种严重影响多种作物的主要害虫。我们的研究通过结合田间药效试验和酶分析来评估甲氨基阿维菌素苯甲酸盐与其他杀虫剂（虱螨脲、氯氰菊酯、毒死蜱和多杀菌素）混合对……的影响，从而提供了新的见解。我们工作的目的是研究五种杀虫剂，即甲氨基阿维菌素苯甲酸盐、虱螨脲、氯氰菊酯、毒死蜱和多杀菌素，在连续两个季节（2023 - 2024年）的田间条件下控制这种害虫的效果。每种杀虫剂均按推荐剂量单独施用，而有些则与甲氨基阿维菌素苯甲酸盐按其推荐剂量的一半混合施用。结果表明，甲氨基阿维菌素苯甲酸盐是最有效的杀虫剂，其次是虱螨脲。评估了甲氨基阿维菌素苯甲酸盐（LC）及其与其他杀虫剂（毒死蜱、多杀菌素、氯氰菊酯和虱螨脲）在不同浓度（LC）下对二龄和四龄……幼虫的联合作用。结果显示与毒死蜱、虱螨脲和氯氰菊酯有相加作用，而与氯氰菊酯（LC）和毒死蜱（LC）有增效作用。在甲氨基阿维菌素苯甲酸盐与多杀菌素（LC和LC）的组合中观察到拮抗作用。本研究得出结论，对于棉花作物中棉铃虫的防治，单独施用杀虫剂更具成本效益。此外，酶活性分析表明，用不同杀虫剂组合处理的幼虫中，α - 酯酶、β - 酯酶、羧酸酯酶、乙酰胆碱酯酶和谷胱甘肽S - 转移酶水平发生了显著变化。
