Fadel M A, Mohamed S A, Abdelbacki A M, El-Sharkawy A H
Biophysics Department, Faculty of Science, Cairo University, Cairo, Egypt.
J Appl Microbiol. 2014 Aug;117(2):358-65. doi: 10.1111/jam.12527. Epub 2014 Jun 10.
Typhoid is a serious disease difficult to be treated with conventional drugs. The aim of this study was to demonstrate a new method for the control of Salmonella typhi growth, through the interference with the bioelectric signals generated from the microbe during cell division by extremely low frequency electromagnetic waves (ELF-EMW-ELF-EM) at resonance frequency.
Isolated Salmonella typhi was subjected to square amplitude modulated waves (QAMW) with different modulation frequencies from two generators with constant carrier frequency of 10 MHz, amplitude of 10 Vpp, modulating depth ± 2 Vpp and constant field strength of 200 V m(-1) at 37°C. Both the control and exposed samples were incubated at the same conditions during the experiment. The results showed that there was highly significant inhibition effect for Salm. typhi exposed to 0·8 Hz QAMW for a single exposure for 75 min. Dielectric relaxation, TEM and DNA results indicated highly significant changes in the molecular structure of the DNA and cellular membrane resulting from the exposure to the inhibiting EM waves.
It was concluded that finding out the inhibiting resonance frequency of ELF-EM waves that deteriorates Salm. typhi growth will be promising method for the treatment of Salm. typhi infection either in vivo or in vitro.
This new non-invasive technique for treatment of bacterial infections is of considerable interest for the use in medical and biotechnological applications.
伤寒是一种难以用传统药物治疗的严重疾病。本研究的目的是展示一种控制伤寒沙门氏菌生长的新方法，即通过极低频电磁波（ELF-EMW-ELF-EM）在共振频率下干扰微生物在细胞分裂过程中产生的生物电信号。
将分离出的伤寒沙门氏菌置于来自两个发生器的不同调制频率的方波调幅波（QAMW）下，载波频率恒定为10MHz，幅度为10Vpp，调制深度±2Vpp，在37°C下场强恒定为200Vm(-1)。在实验过程中，对照样品和暴露样品均在相同条件下孵育。结果表明，暴露于0·8Hz QAMW单次75分钟对伤寒沙门氏菌有高度显著的抑制作用。介电弛豫、透射电子显微镜（TEM）和DNA结果表明，暴露于抑制性电磁波导致DNA和细胞膜的分子结构发生高度显著变化。
得出结论，找出使伤寒沙门氏菌生长恶化的极低频电磁波的抑制共振频率将是体内或体外治疗伤寒沙门氏菌感染的有前景的方法。
这种治疗细菌感染的新的非侵入性技术在医学和生物技术应用中具有相当大的应用价值。
