Akada S, Dube S K
Center for Agricultural Biotechnology, University of Maryland, College Park 20742, USA.
Plant Mol Biol. 1995 Oct;29(2):189-99. doi: 10.1007/BF00043645.
Chalcone synthase (CHS; EC 2.3.1.74), the first committed enzyme of the multibranched pathway of flavonoid/isoflavonoid biosynthesis is encoded by a multigene family in soybean, (Glycine max L. Merrill). Our results suggest that this gene family comprises at least seven members, some of which are clustered. We have identified four chs clusters in the allo-tetraploid G. max genome and chs5, a newly characterized member of the chs gene family is present in two of them. We describe the complete nucleotide sequence of chs5, the identification of its immediate neighbors and the organization of the four hitherto identified chs clusters in the Gm genome.
查尔酮合酶（CHS；EC 2.3.1.74）是类黄酮/异黄酮生物合成多分支途径中的首个关键酶，由大豆（Glycine max L. Merrill）中的一个多基因家族编码。我们的结果表明，该基因家族至少包含七个成员，其中一些成员成簇分布。我们在异源四倍体大豆基因组中鉴定出了四个查尔酮合酶基因簇，查尔酮合酶基因家族的一个新鉴定成员chs5存在于其中两个基因簇中。我们描述了chs5的完整核苷酸序列、其紧邻基因的鉴定以及迄今在大豆基因组中鉴定出的四个查尔酮合酶基因簇的结构。
