Sawaki Yoshiharu, Kobayashi Yuriko, Kihara-Doi Tomonori, Nishikubo Nobuyuki, Kawazu Tetsu, Kobayashi Masatomo, Kobayashi Yasufumi, Iuchi Satoshi, Koyama Hiroyuki, Sato Shigeru
Forestry Research Institute, Oji Paper Co., Ltd., 24-9 Nobono-Cho, Kameyama, Mie 519-0212, Japan.
Forestry Research Institute, Oji Paper Co., Ltd., 24-9 Nobono-Cho, Kameyama, Mie 519-0212, Japan; Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
Plant Sci. 2014 Jun;223:8-15. doi: 10.1016/j.plantsci.2014.02.011. Epub 2014 Mar 5.
Tolerance to soil acidity is an important trait for eucalyptus clones that are introduced to commercial forestry plantations in pacific Asian countries, where acidic soil is dominant in many locations. A conserved transcription factor regulating aluminum (Al) and proton (H⁺) tolerance in land-plant species, STOP1 (SENSITIVE TOPROTON RHIZOTOXICITY 1)-like protein, was isolated by polymerase chain reaction-based cloning, and then suppressed by RNA interference in hairy roots produced by Agrobacterium rhizogenes-mediated transformation. Eucalyptus STOP1-like protein complemented proton tolerance in an Arabidopsis thaliana stop1-mutant, and localized to the nucleus in a transient assay of a green fluorescent protein fusion protein expressed in tobacco leaves by Agrobacterium tumefaciens-mediated transformation. Genes encoding a citrate transporting MULTIDRUGS AND TOXIC COMPOUND EXTRUSION protein and an orthologue of ALUMINUM SENSITIVE 3 were suppressed in transgenic hairy roots in which the STOP1 orthologue was knocked down. In summary, we identified a series of genes for Al-tolerance in eucalyptus, including a gene for STOP1-like protein and the Al-tolerance genes it regulates. These genes may be useful for molecular breeding and genomic selection of elite clones to introduce into acid soil regions.
对于引入到亚洲太平洋国家商业人工林种植的桉树无性系而言，耐土壤酸度是一项重要性状，在这些国家的许多地方酸性土壤占主导地位。通过基于聚合酶链反应的克隆技术，分离出一种在陆地植物物种中保守的调控铝（Al）和质子（H⁺）耐受性的转录因子——类STOP1（对质子根毒性敏感1）蛋白，然后通过发根农杆菌介导的转化在毛状根中利用RNA干扰对其进行抑制。桉树类STOP1蛋白在拟南芥stop1突变体中补充了质子耐受性，并通过根癌农杆菌介导的转化在烟草叶片中表达绿色荧光蛋白融合蛋白的瞬时试验中定位于细胞核。在敲低STOP1直系同源基因的转基因毛状根中，编码柠檬酸转运多药和有毒化合物外排蛋白以及铝敏感3直系同源物的基因受到抑制。总之，我们鉴定出了一系列桉树耐铝基因，包括一个类STOP1蛋白基因及其调控的耐铝基因。这些基因可能有助于对优良无性系进行分子育种和基因组选择，以便引入酸性土壤地区。
