Kim J M, Kim J H, Lee S-Y, Park J B, Kwon C H David, Kim S J, Joh J-W, Lee S-K, Kang E-S
Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
Department of Laboratory Medicine, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
Transplant Proc. 2014 Apr;46(3):861-4. doi: 10.1016/j.transproceed.2013.11.038.
Biliary tract complications are the main concern associated with living-donor liver transplantation (LDLT). Many laboratory parameters have been studied for the early detection of post-LDLT complications, including various cytokines. To explore immunologic activation status and its clinical significance, the cytokine secretion patterns of LDLT patients who developed biliary complications were analyzed. Serum samples from LDLT recipients were collected 1 day before and 3, 7, 14, and 30 days after transplantation. Each sample was tested for interleukin (IL) 2, IL-4, IL-6, IL-8, IL-10, IL-12, interferon-α, and tumor necrosis factor α with the use of multiplex bead flow cytometry. Fifteen patients without any complications and 6 patients with biliary complications showed differential serum cytokine profiles. The biliary complication group (4 biliary stricture and 2 biliary obstruction patients) displayed significantly increased concentrations of IL-2 and IL-12 on post-transplantation days 3 and 7 and of IL-4 on post-transplantation day 7. Profiling cytokine secretion in the serum of patients in the first month of LDLT may be helpful for the prediction and diagnosis of biliary complications within 1 year.
胆道并发症是活体肝移植（LDLT）相关的主要问题。为了早期发现LDLT术后并发症，人们研究了许多实验室指标，包括各种细胞因子。为了探讨免疫激活状态及其临床意义，对发生胆道并发症的LDLT患者的细胞因子分泌模式进行了分析。在移植前1天以及移植后3、7、14和30天收集LDLT受者的血清样本。使用多重微珠流式细胞术对每个样本检测白细胞介素（IL）-2、IL-4、IL-6、IL-8、IL-10、IL-12、干扰素-α和肿瘤坏死因子α。15例无任何并发症的患者和6例有胆道并发症的患者表现出不同的血清细胞因子谱。胆道并发症组（4例胆道狭窄和2例胆道梗阻患者）在移植后第3天和第7天IL-2和IL-12浓度显著升高，在移植后第7天IL-4浓度显著升高。分析LDLT术后第一个月患者血清中的细胞因子分泌情况可能有助于预测和诊断1年内的胆道并发症。
