牛肠系膜淋巴管中的P物质和胃泌素释放肽：化学特性与作用

Substance P and gastrin releasing peptide in bovine mesenteric lymphatic vessels: chemical characterization and action.

作者信息

Foy W L, Allen J M, McKillop J M, Goldsmith J P, Johnston C F, Buchanan K D

机构信息

Department of Medicine, Queens University of Belfast, Ireland, U.K.

出版信息

Peptides. 1989 May-Jun;10(3):533-7. doi: 10.1016/0196-9781(89)90138-1.

DOI:10.1016/0196-9781(89)90138-1

PMID:2476787

Abstract

Alcoholic extracts of bovine mesenteric lymphatic vessels were assayed for the presence of SP, GRP, VIP, PHI, GIP and NT using specific radioimmunoassays. SP and GRP immunoreactivities were detected at concentrations of 190 +/- 20 and 1,000 +/- 130 pg.g-1, respectively. No significant levels of immunoreactivity were detected for any of the other peptides. SP and GRP immunoreactivities coeluted with their synthetic counterparts from both Sephadex G-50 and reversed phase HPLC columns. Synthetic SP (10(-9)-10(-7) M) and the naturally occurring analogue of GRP, bombesin (10(-9)-10(-7) M), increased spontaneous contraction rate in isolated vessel segments. This excitatory effect was not blocked by the alpha-adrenoceptor antagonist phentolamine (3 x 10(-6) M).

摘要

使用特异性放射免疫分析法检测牛肠系膜淋巴管的酒精提取物中P物质（SP）、胃泌素释放肽（GRP）、血管活性肠肽（VIP）、胰高血糖素样肽I（PHI）、葡萄糖依赖性促胰岛素多肽（GIP）和神经降压素（NT）的存在情况。分别在浓度为190±20和1000±130 pg·g-1时检测到SP和GRP免疫反应性。未检测到其他任何肽的显著免疫反应性水平。SP和GRP免疫反应性与它们的合成对应物在Sephadex G-50和反相高效液相色谱柱上共洗脱。合成的SP（10-9 - 10-7 M）和GRP的天然类似物蛙皮素（10-9 - 10-7 M）增加了离体血管段的自发收缩率。这种兴奋作用未被α-肾上腺素能受体拮抗剂酚妥拉明（3×10-6 M）阻断。