Orito T, Shinohara H, Okada Y, Mori M
Department of Oral Surgery, Asahi University School of Dentistry, Gifu, Japan.
Pathol Res Pract. 1989 Jun;184(6):600-8. doi: 10.1016/S0344-0338(89)80165-7.
Immunohistochemical expressions of keratin polypeptides detected by monoclonal antibodies were described in tumor cells of adenolymphoma, and the possibility of intercalated duct and ductal basal cells in the salivary glands being the progenitors was discussed. Basal cells in the tumor showed positive staining for keratin nos. 8, 13, 16, 18 and 19 detecting for monoclonal keratin antibodies (PKK 1, K 4.62, K 8.12, K 8.13), columnar tumor cells displayed strongly positive reactions with RPN 1164 and K4.62 suggesting keratin nos. 8 and 19. Great heterogeneity of distribution for keratin polypeptides was displayed by epithelial cells of adenolymphoma. Intercalated duct cells of normal salivary glands reacted with RPN 1164, RPN 1165, K 4.62 and K 8.13 monoclonal antibodies, which indicates the presence of keratins 8 and 19; and ductal basal cells reacted with PKK 1, K 4.62 and K 8.12, suggesting nos. 8, 13, 16, 18 and 19 keratins. Distribution of involucrin was variable in tumor epithelium of adenolymphoma, and was negative in the normal gland. The immunohistochemical distribution of keratin types between basal tumor cells of adenolymphoma and ductal basal cells of the normal salivary gland was compared.
用单克隆抗体检测了腺淋巴瘤肿瘤细胞中角蛋白多肽的免疫组化表达，并讨论了唾液腺闰管和导管基底细胞作为祖细胞的可能性。肿瘤中的基底细胞对检测单克隆角蛋白抗体（PKK 1、K 4.62、K 8.12、K 8.13）的角蛋白8、13、16、18和19呈阳性染色，柱状肿瘤细胞与RPN 1164和K4.62呈强阳性反应，提示角蛋白8和19。腺淋巴瘤上皮细胞对角蛋白多肽的分布显示出很大的异质性。正常唾液腺的闰管细胞与RPN 1164、RPN 1165、K 4.62和K 8.13单克隆抗体发生反应，这表明存在角蛋白8和19；导管基底细胞与PKK 1、K 4.62和K 8.12发生反应，提示存在角蛋白8、13、16、18和19。在腺淋巴瘤的肿瘤上皮中，内披蛋白的分布是可变的，而在正常腺体中则为阴性。比较了腺淋巴瘤肿瘤基底细胞和正常唾液腺导管基底细胞之间角蛋白类型的免疫组化分布。
