Mori M, Yamada K, Tanaka T, Okada Y
Department of Oral Surgery, Asahi University School of Dentistry, Gifu, Japan.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1990;58(6):435-44. doi: 10.1007/BF02890103.
Immunohistochemical staining for S-100 protein and the intermediate filaments keratin and vimentin, was made in 41 salivary adenomas. In pleomorphic adenomas, great heterogeneity in the staining, as well as multiple and co-expressions of these proteins were found in the outer tumor cells of tubulo-ductal structures and modified myoepithelial cells, but not in the luminal tumor cells. All the outer tumor cells stained for S-100 protein, 97% for K8.12 keratin and 85% for vimentin. Of these cells, 29% showed multiple expression of K8.12 keratin, vimentin, and S-100 protein, and 17% showed co-expression of K8.12 and S-100 protein. Modified and neoplastic myoepithelial cells showed similar expressions of these proteins to those of outer tumor cells; myoepithelioma cells displayed the most complicated pattern, being positive for KL1, PKK1, and K8.12 keratins, vimentin and S-100 protein. In luminal tumor cells there was a heterogeneous expression of KL1 and PKK1 in 82%, and of KL1, PKK1, and K8.12 in only 14.7%. Based on the immunohistochemical findings obtained with different monoclonal antibodies in pleomorphic salivary adenomas, outer tumor cells may be derived from ductal basal cells and luminal tumor cells from intercalated duct cells.
对41例涎腺腺瘤进行了S-100蛋白、中间丝角蛋白和波形蛋白的免疫组织化学染色。在多形性腺瘤中，在管状导管结构的外层肿瘤细胞和改良肌上皮细胞中发现这些蛋白染色存在很大异质性，以及多种蛋白共表达，但管腔内肿瘤细胞中未发现。所有外层肿瘤细胞S-100蛋白染色阳性，97%的细胞K8.12角蛋白染色阳性，85%的细胞波形蛋白染色阳性。在这些细胞中，29%的细胞显示K8.12角蛋白、波形蛋白和S-100蛋白的多种表达，17%的细胞显示K8.12和S-100蛋白的共表达。改良的肿瘤性肌上皮细胞与外层肿瘤细胞显示出相似的蛋白表达；肌上皮瘤细胞显示出最复杂的模式，对KL1、PKK1和K8.12角蛋白、波形蛋白和S-100蛋白呈阳性。在管腔内肿瘤细胞中，82%的细胞KL1和PKK1表达异质性，仅14.7%的细胞KL1、PKK1和K8.12表达异质性。基于在多形性涎腺腺瘤中使用不同单克隆抗体获得的免疫组织化学结果，外层肿瘤细胞可能来源于导管基底细胞，而管腔内肿瘤细胞来源于闰管细胞。
