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胰岛素/胰岛素样生长因子-1信号通路的减弱可恢复秀丽隐杆线虫Piwi突变体生殖细胞的永生性。

Reduced insulin/IGF-1 signaling restores germ cell immortality to Caenorhabditis elegans Piwi mutants.

作者信息

Simon Matt, Sarkies Peter, Ikegami Kohta, Doebley Anna-Lisa, Goldstein Leonard D, Mitchell Jacinth, Sakaguchi Aisa, Miska Eric A, Ahmed Shawn

机构信息

Department of Genetics, University of North Carolina, Chapel Hill, NC 27599, USA; Department of Biology, University of North Carolina, Chapel Hill, NC 27599, USA; Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, NC 27599, USA.

The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.

出版信息

Cell Rep. 2014 May 8;7(3):762-73. doi: 10.1016/j.celrep.2014.03.056. Epub 2014 Apr 24.

DOI:10.1016/j.celrep.2014.03.056
PMID:24767993
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4049074/
Abstract

Defects in the Piwi/piRNA pathway lead to transposon desilencing and immediate sterility in many organisms. We found that the C. elegans Piwi mutant prg-1 became sterile after growth for many generations. This phenotype did not occur for RNAi mutants with strong transposon-silencing defects and was separable from the role of PRG-1 in transgene silencing. Brief periods of starvation extended the transgenerational lifespan of prg-1 mutants by stimulating the DAF-16/FOXO longevity transcription factor. Constitutive activation of DAF-16 via reduced daf-2 insulin/IGF-1 signaling immortalized prg-1 strains via RNAi proteins and histone H3 lysine 4 demethylases. In late-generation prg-1 mutants, desilencing of repetitive segments of the genome occurred, and silencing of repetitive loci was restored in prg-1; daf-2 mutants. This study reveals an unexpected interface between aging and transgenerational maintenance of germ cells, where somatic longevity is coupled to a genome-silencing pathway that promotes germ cell immortality in parallel to the Piwi/piRNA system.

摘要

Piwi/piRNA 通路的缺陷会导致许多生物体中的转座子去沉默并立即不育。我们发现，秀丽隐杆线虫的 Piwi 突变体 prg-1 在生长许多代后会变得不育。这种表型在具有强烈转座子沉默缺陷的 RNAi 突变体中不会出现，并且与 PRG-1 在转基因沉默中的作用是可分离的。短暂的饥饿期通过刺激 DAF-16/FOXO 长寿转录因子延长了 prg-1 突变体的跨代寿命。通过降低 daf-2 胰岛素/IGF-1 信号传导对 DAF-16 的组成型激活通过 RNAi 蛋白和组蛋白 H3 赖氨酸 4 去甲基化酶使 prg-1 菌株永生化。在晚期 prg-1 突变体中，基因组的重复片段发生去沉默，并且在 prg-1;daf-2 突变体中重复位点的沉默得以恢复。这项研究揭示了衰老与生殖细胞跨代维持之间意想不到的联系，即体细胞长寿与一个基因组沉默通路相关联，该通路在与 Piwi/piRNA 系统平行的情况下促进生殖细胞的永生。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/34f08d7fac48/nihms582825f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/b5906781017f/nihms582825f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/ddeff972c355/nihms582825f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/ee2c84919288/nihms582825f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/d00bfe0cbb48/nihms582825f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/7b39b3ea0451/nihms582825f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/34f08d7fac48/nihms582825f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/b5906781017f/nihms582825f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/ddeff972c355/nihms582825f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/ee2c84919288/nihms582825f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/d00bfe0cbb48/nihms582825f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/7b39b3ea0451/nihms582825f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6944/4049074/34f08d7fac48/nihms582825f6.jpg

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2
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Nat Rev Genet. 2012 Oct;13(10):693-704. doi: 10.1038/nrg3246. Epub 2012 Sep 11.
3
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4
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