Reduced insulin/IGF-1 signaling and loss of promote repetitive DNA silencing in .

Non-coding small RNAs and Argonaute proteins mediate conserved defenses against foreign genetic elements. mutants in the insulin/IGF-1 signaling (IIS) have previously been shown to exhibit an enhanced response to exogenous RNAi. Here, we found that the loss of IIS via enhances transgene silencing, which is reversed by knocking out . Similarly, mutants show enhanced RNAi and upregulation of antiviral RNAi pathway. and mutations exhibit additive effects, and loss of restores transgene expression in mutants but not in mutants, suggesting that these genes act in parallel. RNAi gene expression in mutants lacked a consistent pattern, suggesting IIS may regulate RNAi components via post-translational mechanisms.