Mohammadi A, Nazari H, Imani S, Amrollahi H
Department of biochemistry, faculty of medicine, Semnan university of medical sciences, Semnan, Iran.
Department of microbiology, faculty of medicine, Semnan university of medical sciences, Semnan, Iran.
J Mycol Med. 2014 Jun;24(2):e1-8. doi: 10.1016/j.mycmed.2014.02.006. Epub 2014 Apr 24.
The use of and search for drugs and dietary supplements derived from plants have accelerated in recent years. Ethnopharmacologists, botanists, microbiologists and natural-products scientists are combing the earth for phytochemicals and leads, which could be developed for treatment of infectious diseases. The aim of this study was to investigate the antifungal activities of the essential oils of some medicinal plants such as Stachys pubescens, Thymus kotschyanus, Thymus daenensis and Bupleurum falcatum against Fusarium oxysporum, Aspergillus flavus and Alternaria alternata. The essential oils were used to evaluate their MICs and MFCs compared to the amphotricin B as a standard drug. The essential oils were also analyzed by GC/MS. Essential oils isolated from the S. pubescens, T. kotschyanus and B. falcatum showed strong antifungal activities. The essential oil of T. daenensis exhibited a moderate activity against the selected fungi in comparison with the other plants' essential oils. In addition, the results showed that 26, 23, 22 and 15 components were identified from the essential oils of T. kotschyanus, S. pubescens, T. daenensis and B. falcatum, respectively. These oils exhibited a noticeable antifungal activity against the selected fungi. Regarding obtained results and that natural antimicrobial substances are inexpensive and have fewer side effects, they convey potential for implementation in fungal pathogenic systems.
近年来，对源自植物的药物和膳食补充剂的使用及研究加速发展。民族药理学家、植物学家、微生物学家和天然产物科学家正在全球范围内搜寻可用于治疗传染病的植物化学物质和线索。本研究的目的是调查一些药用植物如绵毛水苏、科氏百里香、达乌里秦艽和镰叶柴胡的精油对尖孢镰刀菌、黄曲霉和链格孢的抗真菌活性。以两性霉素B作为标准药物，评估这些精油的最低抑菌浓度（MIC）和最低杀菌浓度（MFC）。同时，采用气相色谱/质谱联用（GC/MS）对精油进行分析。从绵毛水苏、科氏百里香和镰叶柴胡中分离出的精油显示出较强的抗真菌活性。与其他植物的精油相比，达乌里秦艽的精油对所选真菌表现出中等活性。此外，结果表明，分别从科氏百里香、绵毛水苏、达乌里秦艽和镰叶柴胡的精油中鉴定出26、23、22和15种成分。这些精油对所选真菌表现出显著的抗真菌活性。鉴于所获结果以及天然抗菌物质价格低廉且副作用较少，它们在真菌致病系统中具有应用潜力。
