Sarfaraz Danial, Rahimmalek Mehdi, Saeidi Ghodratollah, Sabzalian Mohammad Reza
Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, 84156-83111 Isfahan, Iran.
Department of Horticulture, College of Agriculture, Isfahan University of Technology, 84156-83111 Isfahan, Iran.
3 Biotech. 2020 Jul;10(7):289. doi: 10.1007/s13205-020-02274-6. Epub 2020 Jun 4.
In the present study, the diversity of 11 species was assessed using molecular and morphological markers. Essential oil content and morphological traits were also investigated during two agronomic years. The result of the analysis of variance showed considerable differences among morphological traits. In the first and second years, the essential oil content of the species varied from 0.63 to 1.94% and 0.86 to 2.34%, respectively. and showed the highest essential oil content in two agronomic years. In this research, nine ISSR primers were also used to amplify 151 polymorphic bands in 77 accessions belonging to 11 species. Cluster and principal component (PCA) analyses classified the species in three major groups. Among the species, and presented relatively higher genetic distance in comparison with other species. Analysis of molecular variance (AMOVA) revealed that 72.34% of the total variation was belonged to within-species variation, while 27.66% was associated among the species. High gene flow (Nm = 1.11) and genetic differentiation (Gst = 0.31) were also observed among the species. exhibited the highest genetic variation (0.19), polymorphism % (57.69%), and Shannon index (0.29). The STRUCTURE analysis also showed a high admixture of species that might be originated from a high rate of natural hybridization. Finally, based on molecular and morphological information, and can be suggested as good candidate species for further breeding programs in species.
在本研究中，使用分子和形态学标记评估了11个物种的多样性。在两个农艺年份期间还研究了精油含量和形态特征。方差分析结果表明形态特征之间存在显著差异。在第一年和第二年，这些物种的精油含量分别在0.63%至1.94%和0.86%至2.34%之间变化。[物种名称1]和[物种名称2]在两个农艺年份中显示出最高的精油含量。在本研究中，还使用了9个ISSR引物对属于11个物种的77份材料中的151条多态性条带进行扩增。聚类分析和主成分分析（PCA）将这些物种分为三大类。在这些物种中，[物种名称3]和[物种名称4]与其他物种相比呈现出相对较高的遗传距离。分子方差分析（AMOVA）表明，总变异的72.34%属于种内变异，而27.66%与种间变异相关。在这些物种中还观察到了较高的基因流（Nm = 1.11）和遗传分化（Gst = 0.31）。[物种名称5]表现出最高的遗传变异（0.19）、多态性百分比（57.69%）和香农指数（0.29）。STRUCTURE分析还表明[物种名称6]存在高度混合，这可能源于高比率的自然杂交。最后，基于分子和形态学信息，[物种名称7]和[物种名称8]可被建议作为[物种名称9]进一步育种计划的良好候选物种。
