Vaughn D M, Smyth G B, Whitmer W L, Satjawatcharaphong C
Medicinal Biochemistry Laboratory, Scott-Ritchey Research Program, College of Veterinary Medicine, Auburn University, Alabama 36849-5525.
Vet Res Commun. 1989;13(3):237-49. doi: 10.1007/BF00142050.
Small volumes (0.05 ml) of cisterna magna cerebrospinal fluid (CSF) from 23 neurologically normal horses were analysed for the monoamine neurotransmitters dopamine, norepinephrine, epinephrine, serotonin and their metabolites using high pressure liquid chromatography and electrochemical detection. Two metabolites, homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were present in all CSF samples. The deaminated and methylated metabolite of dopamine, HVA, was present at a mean concentration of 42.33 +/- 3.14 ng/ml of CSF. The deaminated metabolite of serotonin, 5-HIAA, was present at a mean concentration of 45.52 +/- 3.65 ng/ml of CSF. A positive correlation was found between the CSF concentrations of HVA and 5-HIAA (r = 0.72, p = 0.0003). The mean ratio of HVA to 5-HIAA concentrations was 1.07 +/- 0.05. The ratios of HVA to 5-HIAA concentrations were found to be more indicative of the serotonergic metabolite 5-HIAA (r = -0.62, p = 0.0016) than the dopaminergic metabolite HVA (r = 0.11, p = 0.60). There was a stronger relationship between the concentrations of 5-HIAA and the ratios of HVA to 5-HIAA in male CSF (r = -0.85, p = 0.0006) than in female CSF (r = -0.54, p = 0.01). Concentrations of CSF neurotransmitter metabolites were evaluated in horses grouped by age (0-4, 5-9 and 10-13 years). The youngest group of horses had a significantly (p = 0.003) greater CSF concentration of HVA than of 5-HIAA. The 10-13-year-old horses had a significantly (p = 0.02) lower mean CSF HVA concentration than the 0-4-year-old horses. No age-related differences in CSF 5-HIAA concentrations were detected.
对23匹神经功能正常的马的小脑延髓池脑脊液（CSF）小体积样本（0.05毫升）进行分析，使用高压液相色谱法和电化学检测法检测其中的单胺类神经递质多巴胺、去甲肾上腺素、肾上腺素、5-羟色胺及其代谢产物。所有脑脊液样本中均存在两种代谢产物，即高香草酸（HVA）和5-羟吲哚乙酸（5-HIAA）。多巴胺的脱氨基和甲基化代谢产物HVA，在脑脊液中的平均浓度为42.33±3.14纳克/毫升。5-羟色胺的脱氨基代谢产物5-HIAA，在脑脊液中的平均浓度为45.52±3.65纳克/毫升。发现脑脊液中HVA和5-HIAA的浓度之间存在正相关（r = 0.72，p = 0.0003）。HVA与5-HIAA浓度的平均比值为1.07±0.05。结果发现，HVA与5-HIAA浓度的比值对5-羟色胺能代谢产物5-HIAA（r = -0.62，p = 0.0016）的指示作用，比对多巴胺能代谢产物HVA（r = 0.11，p = 0.60）的指示作用更强。在雄性脑脊液中，5-HIAA的浓度与HVA与5-HIAA的比值之间的关系（r = -0.85，p = 0.0006），比在雌性脑脊液中（r = -0.54，p = 0.01）更强。按年龄分组（0至4岁、5至9岁和10至13岁）对马的脑脊液神经递质代谢产物浓度进行评估。最年轻的马组脑脊液中HVA的浓度显著高于5-HIAA（p = 0.003）。10至13岁的马脑脊液中HVA的平均浓度显著低于0至4岁的马（p = 0.02）。未检测到脑脊液中5-HIAA浓度存在与年龄相关的差异。
