Vaughn D M, Smyth G B
Medicinal Biochemistry Laboratory, College of Veterinary Medicine, Auburn University, Alabama 36849-5525.
Vet Res Commun. 1989;13(6):413-9. doi: 10.1007/BF00402561.
The serotonin metabolite, 5-hydroxyindoleacetic acid (5-HIAA) and the dopamine metabolite, homovanillic acid (HVA) in the cerebrospinal fluid (CSF) of seven clinically normal horses were evaluated with reverse phase high pressure liquid chromatography and electrochemical detection. Comparisons of the neurotransmitter metabolite concentrations were made on CSF collected simultaneously from the atlanto-occipital and lumbosacral regions. There were significantly higher amounts of 5-HIAA and HVA in atlanto-occipital CSF than in lumbosacral CSF. Mean 5-HIAA concentrations in atlanto-occipital and lumbosacral CSF were 206.1 +/- 37.0 nmol/L and 68.5 +/- 12.6 nmol/L, respectively. The mean HVA concentration in atlanto-occipital CSF was 254.4 +/- 50.4 nmol/L. No HVA was detected in lumbosacral CSF. In contrast, significantly greater protein concentrations were present in lumbosacral CSF than in atlanto-occipital CSF. Lumbosacral and atlanto-occipital CSF contained 32.1 +/- 3.7 mg protein/dl and 25.0 +/- 1.3 mg protein/dl, respectively. Thus, between the atlanto-occipital and lumbosacral space of the horses, a rostrocaudal gradient existed for neurotransmitter metabolites and a caudorostral gradient existed for protein.
采用反相高压液相色谱法和电化学检测法，对7匹临床正常马匹脑脊液（CSF）中的5-羟色胺代谢物5-羟吲哚乙酸（5-HIAA）和多巴胺代谢物高香草酸（HVA）进行了评估。对同时从寰枕部和腰骶部采集的脑脊液中神经递质代谢物浓度进行了比较。寰枕部脑脊液中5-HIAA和HVA的含量显著高于腰骶部脑脊液。寰枕部和腰骶部脑脊液中5-HIAA的平均浓度分别为206.1±37.0 nmol/L和68.5±12.6 nmol/L。寰枕部脑脊液中HVA的平均浓度为254.4±50.4 nmol/L。在腰骶部脑脊液中未检测到HVA。相反，腰骶部脑脊液中的蛋白质浓度显著高于寰枕部脑脊液。腰骶部和寰枕部脑脊液中的蛋白质含量分别为32.1±3.7 mg/dl和25.0±1.3 mg/dl。因此，在马匹的寰枕部和腰骶部之间，神经递质代谢物存在头尾梯度，蛋白质存在尾头梯度。
