用多克隆抗血清对犬上皮组织进行角蛋白染色。

Keratin staining of canine epithelial tissues by a polyclonal antiserum.

作者信息

Vos J H, van den Ingh T S, Misdorp W, Ramaekers F C, van Mil F N, de Neijs M

出版信息

Zentralbl Veterinarmed A. 1989 May;36(5):374-85. doi: 10.1111/j.1439-0442.1989.tb00743.x.

DOI:10.1111/j.1439-0442.1989.tb00743.x

Abstract

The keratin distribution pattern in various canine epithelial tissues has been studied using a commercially available rabbit antiserum, raised against human skin keratins, in an immunoperoxidase staining method with the peroxidase-antiperoxidase complex (PAP). The staining results of two fixation methods were compared. Paraffin sections after fixation in Carnoy's solution showed optimal results, whereas paraffin sections after fixation in 10% buffered formalin resulted in a strongly reduced keratin staining reaction. Keratinizing and non-keratinizing stratified epithelial showed a strong staining reaction. Glandular epithelium and the non-stratified epithelia of internal organs e.g. liver, kidney and intestine did not react with the antiserum. However, glandular ductal epithelium, myoepithelial cells and basal cells in various epithelial tissues showed a positive staining reaction. The results indicate the presence of different keratin types in these canine epithelial tissues. The keratin distribution pattern is compared with the distribution pattern observed in tissues of other species.

摘要

利用一种市售的、针对人皮肤角蛋白产生的兔抗血清，采用过氧化物酶-抗过氧化物酶复合物（PAP）免疫过氧化物酶染色法，研究了各种犬上皮组织中的角蛋白分布模式。比较了两种固定方法的染色结果。用卡诺氏液固定后的石蜡切片显示出最佳结果，而用10%缓冲福尔马林固定后的石蜡切片导致角蛋白染色反应大大减弱。角化和非角化复层上皮显示出强烈的染色反应。腺上皮以及肝脏、肾脏和肠道等内脏器官的非复层上皮不与抗血清发生反应。然而，各种上皮组织中的腺导管上皮、肌上皮细胞和基底细胞显示出阳性染色反应。结果表明这些犬上皮组织中存在不同类型的角蛋白。将角蛋白分布模式与在其他物种组织中观察到的分布模式进行了比较。