Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, PR China.
Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, PR China; Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, PR China.
Int J Biol Macromol. 2014 Jul;68:92-7. doi: 10.1016/j.ijbiomac.2014.04.029. Epub 2014 Apr 24.
Chitosan and sericin are natural and low cost biomaterials. Both biomaterials displayed good compatibility to human tissues and antibacterial properties for biomedical application. In this study, we have successfully fabricated chitosan/sericin composite nanofibers by electrospinning. The obtained composite nanofibers were characterized using scanning electron microscopy (SEM), Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA) studies. The composite nanofibers had good morphology with diameter between 240nm and 380nm. In vitro methyl thiazolyl tetrazolium (MTT) assays demonstrated that the chitosan/sericin composite nanofibers were biocompatible and could promote the cell proliferation. Furthermore, the composite nanofibers showed good bactericidal activity against both of Gram-positive and Gram-negative bacteria. Thus, the chitosan/sericin composite nanofibers are promising for wound dressing applications.
壳聚糖和丝胶是天然的、低成本的生物材料。这两种生物材料对人体组织具有良好的相容性和抗菌性能，可应用于生物医学领域。在本研究中，我们通过静电纺丝成功制备了壳聚糖/丝胶复合纳米纤维。采用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）、X 射线衍射（XRD）和热重分析（TGA）对所得复合纳米纤维进行了表征。复合纳米纤维形态良好，直径在 240nm 至 380nm 之间。体外噻唑蓝（MTT）分析表明，壳聚糖/丝胶复合纳米纤维具有良好的生物相容性，并能促进细胞增殖。此外，复合纳米纤维对革兰氏阳性菌和革兰氏阴性菌均具有良好的杀菌活性。因此，壳聚糖/丝胶复合纳米纤维有望用于伤口敷料。
