Xiang Hui, Wu Runtian, Xiao Man, An Jianhui, Shang Longchen, Tao Yexing, Deng Lingli
Hubei Key Laboratory of Biological Resources Protection and Utilization, Hubei Minzu University, Enshi 445002, China.
College of Biological and Food Engineering, Hubei Minzu University, Enshi 445002, China.
Biomolecules. 2025 Jun 18;15(6):891. doi: 10.3390/biom15060891.
This study investigated gelatin/zein nanofibers loaded with dihydromyricetin (0-20%, relative to protein weight), before and after the Maillard reaction (60 °C with 50% relative humidity for 6 h). Scanning electron microscopy and diameter distribution analysis indicated that dihydromyricetin incorporation increased the fiber diameter from 692 ± 133 to 922 ± 121 nm, while the nanofibers maintained a uniform morphology following the Maillard reaction. Fourier transform infrared spectroscopy revealed that dihydromyricetin formed hydrogen bonds with protein molecules. X-ray diffraction results indicate that dihydromyricetin was uniformly dispersed within the gelatin/zein nanofibers. The addition of dihydromyricetin improved the thermal stability of the nanofibers. Furthermore, after the Maillard reaction, the nanofibers with dihydromyricetin demonstrated enhanced water resistance. Mechanical testing revealed that nanofibers containing 20% dihydromyricetin after the Maillard reaction exhibited a considerably higher elastic modulus of approximately 90 MPa. In addition, nanofibers containing dihydromyricetin exhibited notable antioxidant activity and antibacterial properties against and . In summary, gelatin/zein nanofibers containing high concentrations of dihydromyricetin exhibited favorable physical and functional properties, supporting their suitability as effective delivery systems for dihydromyricetin in active packaging applications.
本研究调查了在美拉德反应（60°C，相对湿度50%，持续6小时）前后，负载二氢杨梅素（相对于蛋白质重量为0 - 20%）的明胶/玉米醇溶蛋白纳米纤维。扫描电子显微镜和直径分布分析表明，二氢杨梅素的掺入使纤维直径从692±133纳米增加到922±121纳米，而纳米纤维在美拉德反应后保持均匀形态。傅里叶变换红外光谱显示二氢杨梅素与蛋白质分子形成了氢键。X射线衍射结果表明二氢杨梅素均匀分散在明胶/玉米醇溶蛋白纳米纤维内。二氢杨梅素的添加提高了纳米纤维的热稳定性。此外，美拉德反应后，含二氢杨梅素的纳米纤维表现出增强的耐水性。力学测试表明，美拉德反应后含20%二氢杨梅素的纳米纤维表现出约90 MPa的显著更高弹性模量。此外，含二氢杨梅素的纳米纤维对[具体菌种1]和[具体菌种2]表现出显著的抗氧化活性和抗菌性能。总之，含有高浓度二氢杨梅素的明胶/玉米醇溶蛋白纳米纤维表现出良好的物理和功能特性，支持它们作为二氢杨梅素在活性包装应用中的有效递送系统的适用性。
