Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario N6A 5B9, Canada.
Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario N6A 5B9, Canada; The Graduate Program of Biomedical Engineering, The University of Western Ontario, London, Ontario N6A 5B9, Canada; Department of Chemistry, The University of Western Ontario, London, Ontario N6A 5B7, Canada.
Acta Biomater. 2014 Aug;10(8):3484-96. doi: 10.1016/j.actbio.2014.04.014. Epub 2014 Apr 24.
Functionalization of polymeric biomaterials permits the conjugation of cell signaling molecules capable of directing cell function. In this study, l-phenylalanine and l-aspartic acid were used to synthesize poly(ester amide)s (PEAs) with pendant carboxylic acid groups through an interfacial polycondensation approach. Human coronary artery smooth muscle cell (HCASMC) attachment, spreading and proliferation was observed on all PEA films. Vinculin expression at the cell periphery suggested that HCASMCs formed focal adhesions on the functional PEAs, while the absence of smooth muscle α-actin (SMαA) expression implied the cells adopted a proliferative phenotype. The PEAs were also electrospun to yield nanoscale three-dimensional (3-D) scaffolds with average fiber diameters ranging from 130 to 294nm. Immunoblotting studies suggested a potential increase in SMαA and calponin expression from HCASMCs cultured on 3-D fibrous scaffolds when compared to 2-D films. X-ray photoelectron spectroscopy and immunofluorescence demonstrated the conjugation of transforming growth factor-β1 to the surface of the functional PEA through the pendant carboxylic acid groups. Taken together, this study demonstrates that PEAs containing aspartic acid are viable biomaterials for further investigation in vascular tissue engineering.
聚合物生物材料的功能化允许细胞信号分子的共轭，这些分子能够指导细胞功能。在这项研究中，通过界面缩聚方法，使用 L-苯丙氨酸和 L-天冬氨酸合成了带有侧链羧酸基团的聚（酯酰胺）（PEA）。在所有 PEA 薄膜上都观察到了人冠状动脉平滑肌细胞（HCASMC）的附着、铺展和增殖。细胞边缘的 vinculin 表达表明 HCASMC 在功能化的 PEA 上形成了粘着斑，而平滑肌α-肌动蛋白（SMαA）的缺失表达暗示细胞呈现出增殖表型。PEA 也被电纺成具有平均纤维直径从 130nm 到 294nm 的纳米级三维（3-D）支架。免疫印迹研究表明，与 2-D 薄膜相比，在 3-D 纤维支架上培养的 HCASMC 中 SMαA 和 calponin 的表达可能增加。X 射线光电子能谱和免疫荧光表明转化生长因子-β1 通过侧链羧酸基团接枝到功能化 PEA 的表面。综上所述，这项研究表明，含有天冬氨酸的 PEA 是血管组织工程进一步研究的可行生物材料。
