Aslankoohi Neda, Lin Shigang, Mequanint Kibret
School of Biomedical Engineering, The University of Western Ontario, London, Ontario, N6A 5B9, Canada.
Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario, N6A 5B9, Canada.
Mater Today Bio. 2021 Dec 9;13:100187. doi: 10.1016/j.mtbio.2021.100187. eCollection 2022 Jan.
Osteogenic differentiation of stem cells is one of the essential steps in bone regeneration. While supplementing exogenous factors using differentiation media is the established method to differentiate stem cells into osteoblasts on biomaterials, designing biomaterials that can act as a stand-alone differentiation inducer and promote bone regeneration is preferred for clinical translation. In this work, we report dexamethasone-loaded organic-inorganic hybrid microparticles synthesized from an intrinsically fluorescent poly (ester amide) and tertiary bioactive glass (PEA-BG) as a stand-alone osteogenic differentiation inducer. The mechanical properties data indicated that the compressive modulus of fluorescent hybrid microparticles could be modulated by its composition. The hybrid fluorescent microparticles supported the adhesion and proliferation of 10T1/2 cells in culture for up to seven days. Both pristine and dexamethasone-loaded PEA-BG microparticles were able to induce osteogenic differentiation of 10T1/2 cells in the absence of any media supplement, to a level even higher than standard osteogenic media, as evidenced by the expression of osteogenic markers on gene and protein levels and matrix mineralization. Taken together, the fluorescent PEA-BG hybrid microparticles have the potential to be used as a stand-alone biomaterial for osteogenic differentiation and bone regeneration.
干细胞的成骨分化是骨再生的关键步骤之一。虽然使用分化培养基补充外源性因子是将干细胞在生物材料上分化为成骨细胞的既定方法，但设计一种能够作为独立分化诱导剂并促进骨再生的生物材料对于临床转化更为可取。在这项工作中，我们报道了由具有内在荧光的聚（酯酰胺）和三级生物活性玻璃（PEA-BG）合成的载有地塞米松的有机-无机杂化微粒，作为一种独立的成骨分化诱导剂。力学性能数据表明，荧光杂化微粒的压缩模量可通过其组成进行调节。杂化荧光微粒在培养中支持10T1/2细胞的黏附和增殖长达7天。无论是原始的还是载有地塞米松的PEA-BG微粒，在没有任何培养基补充的情况下都能够诱导10T1/2细胞的成骨分化，其水平甚至高于标准成骨培养基，这在基因和蛋白质水平上成骨标志物的表达以及基质矿化中得到了证实。综上所述，荧光PEA-BG杂化微粒有潜力用作成骨分化和骨再生的独立生物材料。
