Department of Chemistry-BMC, Uppsala University, Box 576, SE 751 23 Uppsala, Sweden.
Curr Opin Chem Biol. 2014 Aug;21:42-7. doi: 10.1016/j.cbpa.2014.03.015. Epub 2014 Apr 24.
Hydrolytic enzymes play important roles as biocatalysts in chemical synthesis. The chemical versatility and structurally sturdy features of Candida antarctica lipase B has placed this enzyme as a common utensil in the synthetic tool-box. In addition to catalyzing acyl transfer reactions, a number of promiscuous activities have been described recently. Some of these new enzyme activities have been amplified by mutagenesis. Epoxide hydrolases are of interest due to their potential as catalysts in asymmetric synthesis. This current update discusses recent development in the engineering of lipases and epoxide hydrolases aiming to generate new biocatalysts with refined features as compared to the wild-type enzymes. Reported progress in improvements in reaction atom economy from dynamic kinetic resolution or enantioconvergence is also included.
水解酶在化学合成中作为生物催化剂发挥着重要作用。南极假丝酵母脂肪酶 B 的化学多功能性和结构坚固性使其成为合成工具箱中的常用工具。除了催化酰基转移反应外，最近还描述了许多混杂的活性。其中一些新的酶活性已通过诱变得到放大。环氧化物水解酶因其在不对称合成中的潜在应用而受到关注。本综述讨论了脂肪酶和环氧化物水解酶的工程改造的最新进展，旨在生成与野生型酶相比具有改良特征的新型生物催化剂。还包括动态动力学拆分或对映体收敛反应中原子经济性提高的报道进展。
