Department of Oral Medicine, Faculty of Medicine and Dentistry. Ageing Research Institute, University of Murcia, Murcia, Spain.
Department of Oral Medicine, Faculty of Medicine and Dentistry. Ageing Research Institute, University of Murcia, Murcia, Spain.
Environ Res. 2014 Jul;132:140-5. doi: 10.1016/j.envres.2014.03.032. Epub 2014 Apr 25.
The aim of this study was to evaluate DNA damage and cytokinetic defects, proliferative potential and cell death caused by the frequent use of mouthrinses containing chlorhexidine, triclosan and essential oils in ethanolic solution, compared to a placebo mouthwash.
This double-blind, prospective, randomized clinical trial included 80 Caucasian patients. Subjects were divided into four groups: Group I used a mouthrinse, Triclosan; Group II used physiological saline; Group III used chlorhexidine; Group IV a mouthrinse with essential oils in ethanolic solution. All subjects used the mouthrinses for two weeks (15 ml, twice a day, rinsing for 30s). Two cell samples per subject were collected, before and after mouthrinse use (on day 0 and day 15). Samples were processed as follows: cell collection from cheeks with a cytobrush; cell centrifuge; slide preparation, fixation and staining; and fluorescent microscope analysis. 2000 exfoliated cells were screened for nuclear abnormalities, particularly the presence of micronuclei by means of cytome assay.
No significant differences between study times (before and after use of mouthwash) were identified for any of the variables studied (p>0.05). Differences between mouthrinse groups were also compared but no significant differences were found (p>0.05).
This study did not observe any genotoxic effect resulting from mouthrinse use.
本研究旨在评估频繁使用含氯己定、三氯生和精油的漱口水与安慰剂漱口水相比，对 DNA 损伤和细胞有丝分裂缺陷、增殖潜能和细胞死亡的影响。
这是一项双盲、前瞻性、随机临床试验，纳入了 80 名白种人患者。受试者分为四组：组 I 使用含三氯生的漱口水；组 II 使用生理盐水；组 III 使用洗必泰；组 IV 使用含精油的乙醇漱口水。所有受试者均使用漱口水两周（15ml，每天两次，漱口 30s）。在使用漱口水前（第 0 天）和后（第 15 天），每个受试者采集两个细胞样本。样本处理如下：用细胞刷从脸颊上采集细胞；细胞离心；载玻片制备、固定和染色；荧光显微镜分析。通过细胞微核检测法筛选 2000 个脱落细胞，以检测核异常，特别是微核的存在。
在所研究的变量中，任何一个变量在研究时间（使用漱口水前后）之间均无显著差异（p>0.05）。还比较了漱口水组之间的差异，但未发现显著差异（p>0.05）。
本研究未观察到使用漱口水导致的遗传毒性作用。
