Faculty of Dental Medicine, Department of Prosthodontics, Lebanese University, Beirut, Hadat, Lebanon.
Odontology. 2021 Apr;109(2):327-335. doi: 10.1007/s10266-020-00547-3. Epub 2020 Aug 17.
This research has several purposes: First to assess the bacterial and fungal minimum inhibitory concentration of Origanum Syriacum essential oil. Second to quantify its bactericidal and fungicidal minimal concentration against S. aureus, S. mutans, and C. albicans found in denture stomatitis. The third purpose is to look at the influence of three different soils (Annaya, Bhanin and Michrif) on the essential oils composition. Three essential oils were extracted by hydro-distillation from three different Origanum Syriacum plant origins. Bioassays were conducted using a broth microdilution methods. Gas Chromatography analysis was used to calculate the abundance of most components in each essential oil. Post hoc tests assessed antimicrobial effects between ecotypes while Pearson's test correlated the different components and their antimicrobial efficiency (α < 0.05). All tested essential oils were efficient against all microorganisms. Origanum Syriacum essential oils derived from Annaya and Bhanin soils showed a superior antimicrobial activity compared to the Michrif one. The most abundant component and most efficient among all essential oils constituents was carvacrol. It can be concluded that Origanum Syriacum essential oils have an antimicrobial activity, which depends on the ecotype, its origin and its composition. They might be used to start a clinical trial for the treatment of denture stomatitis.
首先，评估牛至精油的细菌和真菌最低抑菌浓度。其次，量化其对义齿性口炎中发现的金黄色葡萄球菌、变形链球菌和白色念珠菌的杀菌和抑菌最小浓度。第三个目的是观察三种不同土壤（Annaya、Bhanin 和 Michrif）对精油成分的影响。从三种不同的牛至植物来源中通过水蒸馏提取了三种精油。采用肉汤微量稀释法进行生物测定。气相色谱分析用于计算每种精油中大多数成分的丰度。事后检验评估了生态型之间的抗菌效果，而皮尔逊检验则关联了不同成分及其抗菌效率（α<0.05）。所有测试的精油对所有微生物都有效。来自 Annaya 和 Bhanin 土壤的牛至精油显示出比 Michrif 土壤更强的抗菌活性。在所有精油成分中，最丰富的成分和最有效的成分是香芹酚。可以得出结论，牛至精油具有抗菌活性，这种活性取决于生态型、其来源及其成分。它们可能被用于启动治疗义齿性口炎的临床试验。
