Suzuki Ryosuke, Konishi Eiji
Department of Virology II, National Institute of Infectious Diseases.
Uirusu. 2013;63(1):13-22. doi: 10.2222/jsv.63.13.
Flaviviruses such as Japanese encephalitis virus, West Nile virus, yellow fever virus, dengue virus, and tick-borne encephalitis virus belong to a family Flaviviridae. These viruses are transmitted to vertebrates by infected mosquitoes or ticks, producing diseases, which have a serious impact on global public health. Reverse genetics is a powerful tool for studying the viruses. Although infectious full-length clones have been obtained for multiple flaviviruses, their early-stage development had the difficulty because of the instability problem of the viral cDNA in E. coli. Several strategies have been developed to circumvent the problem of infectious clone instability. The current knowledge accumulated on reverse genetics system of flaviviruses and its application are summarized in this review.
诸如日本脑炎病毒、西尼罗河病毒、黄热病病毒、登革病毒和蜱传脑炎病毒等黄病毒属于黄病毒科。这些病毒通过受感染的蚊子或蜱传播给脊椎动物，引发对全球公共卫生有严重影响的疾病。反向遗传学是研究这些病毒的有力工具。尽管已经获得了多种黄病毒的感染性全长克隆，但由于病毒cDNA在大肠杆菌中的不稳定性问题，其早期开发存在困难。已经开发了几种策略来规避感染性克隆不稳定性的问题。本文综述了目前在黄病毒反向遗传学系统及其应用方面积累的知识。
