Walker C, Nettesheim P
Laboratory of Pulmonary Pathobiology, National Institute of Environmental Health Science, Research Triangle Park, North Carolina.
Mol Carcinog. 1989;2(3):117-20. doi: 10.1002/mc.2940020302.
Five tumor-derived cell lines established from transformed rat tracheal epithelial (RTE) cells were examined for activated oncogenes using the NIH 3T3 assay, and the expression of 11 cellular oncogenes in the transformed cells was quantitated and compared with expression in normal RTE cells. DNA from the tumor-derived cell lines lacked transforming activity, but expression of several oncogenes (fos, abl, Ki-ras, Ha-ras, and p53) was higher in the transformed cells than in normal RTE cells.
利用NIH 3T3检测法对从转化的大鼠气管上皮（RTE）细胞建立的5种肿瘤衍生细胞系进行了活化癌基因检测，并对转化细胞中11种细胞癌基因的表达进行了定量分析，并与正常RTE细胞中的表达进行了比较。肿瘤衍生细胞系的DNA缺乏转化活性，但几种癌基因（fos、abl、Ki-ras、Ha-ras和p53）在转化细胞中的表达高于正常RTE细胞。
