Cosma G, Hubbard F, Jamasbi R J, Marchok A, Garte S J
Institute of Environmental Medicine, New York University Medical Center, New York 10016.
J Cancer Res Clin Oncol. 1994;120(11):641-4. doi: 10.1007/BF01245374.
The effect of an activated H-ras oncogene on the progression of neoplasia was studied in transformed rat tracheal epithelial cells. Nude mouse tumours produced by injection of these cells exhibited a higher fraction of cells containing the mutant ras gene that did the injected cells, while a subclone that lacked the ras mutation was much less tumorigenic than parental cells. Serial passage of one cell line containing a ras mutation resulted in an increase in the fraction of ras-mutated cells, which suggests that, in this line, ras activation may confer a selective advantage in vitro as well. However, this was not seen in another ras-containing line, suggesting the importance of alternative pathways in malignant progression of rat tracheal epithelial cells.
在转化的大鼠气管上皮细胞中研究了活化的H-ras癌基因对肿瘤形成进程的影响。注射这些细胞产生的裸鼠肿瘤中，含有突变ras基因的细胞比例高于注射的细胞，而缺乏ras突变的亚克隆的致瘤性远低于亲代细胞。一个含有ras突变的细胞系连续传代导致ras突变细胞比例增加，这表明在该细胞系中，ras激活在体外可能也赋予了选择性优势。然而，在另一个含有ras的细胞系中未观察到这种情况，这表明其他途径在大鼠气管上皮细胞恶性进展中具有重要性。
