Shu S Y, Penny G R, Peterson G M
Department of Anatomy and Cell Biology, East Carolina University, School of Medicine, Greenville, North Carolina 27858.
J Chem Neuroanat. 1988 May-Jun;1(3):147-63.
Using a combination of anterograde and retrograde (Phaseolus vulgaris leucoagglutinin; PHA-L and wheat germ agglutinin conjugated horseradish peroxidase; WGA-HRP) tract-tracing methods and histochemical techniques, a new subdivision of the neostriatum, the marginal division, has been found in the rat brain. The marginal division is approximately 120 microns wide and is located at the caudal extent of the neostriatum and surrounds the rostral edge of the globus pallidus. The neuronal somata of the marginal division are mostly fusiform in shape, with their long axes running parallel to the border between the striatum and the globus pallidus. Histochemically, the marginal division is lighter in AChE staining, is more densely filled with Met-enkephalin-immunoreactive terminals, and has fewer choline acetyltransferase (ChAT)-immunoreactive neurons than does the rest of the neostriatum. Injections of PHA-L or WGA-HRP demonstrated that the projections of the marginal division differ from those of the main body of the striatum. The striatopallidal projection from the marginal division terminates in the caudal-most part of the globus pallidus which is rich in cholinergic neurons. In contrast, the projection from the main region of the neostriatum terminates in two bands in the globus pallidus, both of which are rostral to the area of termination of the fibres from the marginal division. The striatonigral fibres from the marginal division terminate in the caudal part of the substantia nigra pars reticulata whereas the rest of neostriatum projects to a more rostral region. Based on its cellular morphology, immunohistochemistry and projection pattern, we conclude that the marginal division of the striatum is a distinct subdivision of the neostriatum.
运用顺行和逆行（菜豆白细胞凝集素；PHA-L和小麦胚凝集素结合辣根过氧化物酶；WGA-HRP）示踪方法与组织化学技术相结合，在大鼠脑中发现了新纹状体的一个新分区——边缘区。边缘区宽约120微米，位于新纹状体的尾端，围绕苍白球的嘴侧边缘。边缘区的神经元胞体大多呈梭形，其长轴与纹状体和苍白球之间的边界平行。组织化学上，边缘区的乙酰胆碱酯酶染色较浅，富含甲硫氨酸脑啡肽免疫反应性终末，且胆碱乙酰转移酶（ChAT）免疫反应性神经元比新纹状体的其他部分少。PHA-L或WGA-HRP注射显示，边缘区的投射与纹状体主体的投射不同。边缘区的纹状体苍白球投射终止于苍白球富含胆碱能神经元的最尾端部分。相比之下，新纹状体主要区域的投射终止于苍白球的两条带，这两条带都在边缘区纤维终止区域的嘴侧。边缘区的纹状体黑质纤维终止于黑质网状部的尾侧部分，而新纹状体的其余部分投射到更靠前的区域。基于其细胞形态、免疫组织化学和投射模式，我们得出结论，纹状体的边缘区是新纹状体的一个独特分区。
