Departamento de Bioquímica e Biologia Molecular and Instituto Nacional de Ciência e Tecnologia em Salinidade (INCTSal/CNPq), Universidade Federal do Ceará, CP 6039, Fortaleza, CE, 60440-970, Brazil.
Plant Cell Rep. 2014 Aug;33(8):1289-306. doi: 10.1007/s00299-014-1616-5. Epub 2014 Apr 26.
Cowpea cultivars differing in salt tolerance reveal differences in protein profiles and adopt different strategies to overcome salt stress. Salt-tolerant cultivar shows induction of proteins related to photosynthesis and energy metabolism. Salinity is a major abiotic stress affecting plant cultivation and productivity. The objective of this study was to examine differential proteomic responses to salt stress in leaves of the cowpea cultivars Pitiúba (salt tolerant) and TVu 2331 (salt sensitive). Plants of both cultivars were subjected to salt stress (75 mM NaCl) followed by a recovery period of 5 days. Proteins extracted from leaves of both cultivars were analyzed by two-dimensional electrophoresis (2-DE) under salt stress and after recovery. In total, 22 proteins differentially regulated by both salt and recovery were identified by LC-ESI-MS/MS. Our current proteome data revealed that cowpea cultivars adopted different strategies to overcome salt stress. For the salt-tolerant cultivar (Pitiúba), increase in abundance of proteins involved in photosynthesis and energy metabolism, such as rubisco activase, ribulose-5-phosphate kinase (Ru5PK) (EC 2.7.1.19), glycine decarboxylase (EC 1.4.4.2) and oxygen-evolving enhancer (OEE) protein 2, was observed. However, these vital metabolic processes were more profoundly affected in salt-sensitive cultivar (TVu), as indicated by the down-regulation of OEE protein 1, Mn-stabilizing protein-II, carbonic anhydrase (EC 4.2.1.1) and Rubisco (EC 4.1.1.39), leading to energy reduction and a decline in plant growth. Other proteins differentially regulated in both cultivars corresponded to different physiological responses. Overall, our results provide information that could lead to a better understanding of the molecular basis of salt tolerance and sensitivity in cowpea plants.
豇豆品种的耐盐性差异揭示了蛋白质图谱的差异，并采用不同的策略来克服盐胁迫。耐盐品种表现出与光合作用和能量代谢相关的蛋白质的诱导。盐度是影响植物栽培和生产力的主要非生物胁迫。本研究的目的是研究盐胁迫下豇豆品种 Pitiúba（耐盐）和 TVu 2331（盐敏感）叶片的差异蛋白质组响应。两种品种的植物均受到盐胁迫（75 mM NaCl），然后进行 5 天的恢复。从两种品种的叶片中提取蛋白质，在盐胁迫和恢复后通过二维电泳（2-DE）进行分析。总共，通过 LC-ESI-MS/MS 鉴定了 22 种由盐和恢复共同调节的差异表达蛋白。我们目前的蛋白质组数据表明，豇豆品种采用不同的策略来克服盐胁迫。对于耐盐品种（Pitiúba），观察到与光合作用和能量代谢相关的蛋白质丰度增加，如 Rubisco 激活酶、核酮糖-5-磷酸激酶（Ru5PK）（EC 2.7.1.19）、甘氨酸脱羧酶（EC 1.4.4.2）和氧释放增强剂（OEE）蛋白 2。然而，这些重要的代谢过程在盐敏感品种（TVu）中受到更严重的影响，如 OEE 蛋白 1、Mn 稳定蛋白-II、碳酸酐酶（EC 4.2.1.1）和 Rubisco（EC 4.1.1.39）的下调所示，导致能量减少和植物生长下降。其他在两种品种中差异调节的蛋白质对应于不同的生理反应。总体而言，我们的结果提供了信息，可能导致对豇豆植物耐盐性和敏感性的分子基础的更好理解。
