Rahman Md Atikur, Alam Iftekhar, Kim Yong-Goo, Ahn Na-Young, Heo Sung-Hyun, Lee Dong-Gi, Liu Gongshe, Lee Byung-Hyun
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Division of Applied Life Sciences (BK21Plus), IALS, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea; National Institute of Biotechnology, Ganakbari, Savar, Dhaka 1349, Bangladesh.
Plant Physiol Biochem. 2015 Apr;89:112-22. doi: 10.1016/j.plaphy.2015.02.015. Epub 2015 Feb 24.
A comparative proteomic approach was carried out between two contrasting alfalfa cultivars, nonomu (NM-801; salt tolerant) and vernal (VN; salt intolerant) in terms of salt tolerance. Seedlings were subjected to salt stress (50 and 100 mM NaCl) for three days. Several physiological parameters (leaf water, chlorophyll, root Na(+), K(+), and Ca(2+)) and root proteome profile were analyzed. Comparison of physiological status revealed that NM-801 is more tolerant to salt than VN. Eighty three differentially expressed proteins were found on 2-DE maps, of which 50 were identified by MALDI-TOF or MALDI-TOF/TOF mass spectrometry. These proteins were involved in ion homeostasis, protein turnover and signaling, protein folding, cell wall components, carbohydrate and energy metabolism, reactive oxygen species regulation and detoxification, and purine and fatty acid metabolism. The comparative proteome analysis showed that 33 salt-responsive proteins were significantly changed in both cultivars, while 17 (14 in VN and 3 in NM-801) were cultivar-specific. Peroxidase, protein disulfide-isomerase, NAD synthetase, and isoflavone reductase were up-regulated significantly only in NM-801 in all salt concentrations. In addition, we identified novel proteins including NAD synthetase and biotin carboxylase-3 that were not reported previously as salt-responsive. Taken together, these results provide new insights of salt stress tolerance in alfalfa.
采用比较蛋白质组学方法，对两个耐盐性不同的苜蓿品种——诺诺木（NM - 801；耐盐）和薇若娜（VN；不耐盐）进行耐盐性研究。将幼苗置于盐胁迫（50和100 mM NaCl）下处理三天。分析了几个生理参数（叶片水分、叶绿素、根中Na(+)、K(+)和Ca(2+)）以及根蛋白质组图谱。生理状态比较表明，NM - 801比VN更耐盐。在二维电泳图谱上发现了83种差异表达蛋白，其中50种通过基质辅助激光解吸电离飞行时间质谱（MALDI - TOF）或串联质谱（MALDI - TOF/TOF）鉴定。这些蛋白质参与离子稳态、蛋白质周转与信号传导、蛋白质折叠、细胞壁成分、碳水化合物和能量代谢、活性氧调节与解毒以及嘌呤和脂肪酸代谢。比较蛋白质组分析表明，33种盐响应蛋白在两个品种中均有显著变化，而17种（VN中14种，NM - 801中3种）是品种特异性的。在所有盐浓度下，过氧化物酶、蛋白质二硫键异构酶、NAD合成酶和异黄酮还原酶仅在NM - 801中显著上调。此外，我们鉴定出了包括NAD合成酶和生物素羧化酶 - 3在内的新蛋白质，这些蛋白质以前未被报道为盐响应蛋白。综上所述，这些结果为苜蓿耐盐胁迫提供了新的见解。
