Kameda Y
Department of Anatomy, Fukuoka University School of Medicine, Japan.
Cell Tissue Res. 1989 Sep;257(3):623-9. doi: 10.1007/BF00221473.
An immunoperoxidase method was used to investigate and compare the distribution of neuropeptide-immunoreactive (ir) nerve fibers and neurofilament-ir fibers in chick carotid body. The vagus nerve and its branches were intensely immunoreactive with an antiserum against chick neurofilaments. The branches from the vagus and the recurrent laryngeal nerves anastomosed within the connective tissue encircling the carotid body, and then entered the organ to form a network of neurofilament-ir fibers. Immunoreactivities for CGRP, somatostatin, galanin, VIP and substance P were found in the carotid body; they were located within varicose fibers. Immunoreactivity for each peptide was discretely and characteristically distributed. Dense networks of varicose CGRP-ir nerve fibers were found throughout the carotid body in close proximity to clusters of carotid body cells and to blood vessels. Substance P-ir fibers were distributed similarly to CGRP-ir fibers. Somatostatin-ir fibers appeared as patches distributed around chief cells. Numerous galanin- and VIP-ir nerve fibers were observed in the connective tissue surrounding the carotid body, but they occurred in only moderate densities in the parenchyma.
采用免疫过氧化物酶法研究并比较鸡颈动脉体中神经肽免疫反应性（ir）神经纤维和神经丝免疫反应性纤维的分布。迷走神经及其分支与抗鸡神经丝抗血清呈现强烈的免疫反应性。迷走神经分支与喉返神经在环绕颈动脉体的结缔组织内吻合，然后进入该器官形成神经丝免疫反应性纤维网络。在颈动脉体中发现了降钙素基因相关肽（CGRP）、生长抑素、甘丙肽、血管活性肠肽（VIP）和P物质的免疫反应性；它们位于曲张纤维内。每种肽的免疫反应性呈离散且特征性分布。在整个颈动脉体中发现密集的曲张CGRP免疫反应性神经纤维网络，紧邻颈动脉体细胞簇和血管。P物质免疫反应性纤维的分布与CGRP免疫反应性纤维相似。生长抑素免疫反应性纤维呈斑块状分布在主细胞周围。在颈动脉体周围的结缔组织中观察到大量甘丙肽和VIP免疫反应性神经纤维，但它们在实质中的密度仅为中等。
