Igarashi Y, Takeshima T, Tauchi K, Tanaka K, Ogawa S
Imamichi Institute for Animal Reproduction, Ibaraki, Japan.
Jikken Dobutsu. 1989 Jul;38(3):263-7. doi: 10.1538/expanim1978.38.3_263.
The fluorescent body (F-body) was identified with quinacrine mustard (Q-M) staining in spermatozoon and lymphocyte of canine. Well washed sperm suspension was treated with protease (125 mg/ml) or dispase (2000p. u./ml) and staining with Q-M (final dilution 50 micrograms/ml) for 15 min to 24 hr at 37 degrees C. The lymphocyte cultures from whole blood were prepared as routine human investigation. The chromosomal preparation made by air dry method was stained with Q-M (final dilution 0.5 to 50 micrograms/ml) after pretreatment of enzyme digestion. The examination using a reflected fluorescent microscope revealed that the same F-body in human was present in both spermatozoon (20.1-39.7%) and interphase of lymphocyte (0.37.2%) of male origin.
用喹吖因氮芥（Q-M）染色法在犬的精子和淋巴细胞中鉴定出荧光小体（F体）。将充分洗涤后的精子悬液用蛋白酶（125毫克/毫升）或分散酶（2000国际单位/毫升）处理，并用Q-M（终浓度50微克/毫升）在37℃下染色15分钟至24小时。全血淋巴细胞培养物按照常规人体研究方法制备。空气干燥法制得的染色体标本在酶消化预处理后用Q-M（终浓度0.5至50微克/毫升）染色。使用反射荧光显微镜检查发现，人类中同样的F体存在于雄性来源的精子（20.1 - 39.7%）和淋巴细胞间期（0.3 - 7.2%）中。
