Ogawa S, Yamakawa H, Yamanoi J, Nishida S, Kano Y, Takeshima T, Tauchi K, Nagashima H
Labolatory of Animal Reproduction, Ikuta-Campus, Meiji University, Higashimita 1-1-1, Tama-ku, Kawasaki 214, Japan.
Theriogenology. 1988;29(5):1083-9. doi: 10.1016/s0093-691x(88)80032-3.
An attempt was made to detect the fluorescent bodies (F-body), using Quinacrine mustard (Q-M) staining in the spermatozoa from eight mammalian species (human, bull, boar, dog, rabbit, rat, mouse, and mastomys) as well as in the cock (used as negative control). Sperm suspension, prepared after rinsing by repeated centrifugation with phosphate buffered saline (PBS), was either stained with Q-M for 24 h or treated with protease and then stained with Q-M for 60 min. The final concentration of Q-M in the mixed staining sperm suspension was 0.025 mg/ml. The examination using a reflecting fluorescent microscope revealed that the F-body found in human sperm was also present in the sperm of all the mammals but not in the cock after 24 h of staining. The enzyme-treated specimens showed higher incidences of F-bodies than specimens stained for 24 h without enzymatic digestion. These findings strongly suggest that the F-body is commonly present in the spermatozoa of many mammalian species.
尝试使用喹吖因氮芥（Q-M）染色法，检测来自八个哺乳动物物种（人类、公牛、公猪、狗、兔子、大鼠、小鼠和非洲沼鼠）的精子中的荧光小体（F小体），并以公鸡（用作阴性对照）进行检测。精子悬浮液经磷酸盐缓冲盐水（PBS）反复离心冲洗后制备，用Q-M染色24小时，或先用蛋白酶处理，再用Q-M染色60分钟。混合染色的精子悬浮液中Q-M的最终浓度为0.025毫克/毫升。使用反射荧光显微镜检查发现，人类精子中发现的F小体在所有哺乳动物的精子中也存在，但在染色24小时后的公鸡精子中未发现。酶处理的标本比未经酶消化染色24小时的标本显示出更高的F小体发生率。这些发现有力地表明，F小体普遍存在于许多哺乳动物物种的精子中。
