Cui Zhi-Qiang, Zhao Na, Zhang Jun-Mian, Chen Mei, Cong Yan-Li, Guo Yu, Zhang Zhen-Guo, Li Qi
Bing Du Xue Bao. 2014 Jan;30(1):33-6.
This study aims to investigate the serotype distribution of non-polio enterovirus (NPEV) isolated from patients with acute flaccid paralysis (AFP) during 2011-2012 in Hebei Province, China and to analyze the relationship between these viruses and AFP. NPEV strains were isolated from the stool specimens from AFP cases in Hebei using human rhabdomyosarcoma cells (RD) and the mouse cell line expressing the gene for the human cellular receptor for poliovirus (L20B) according to the WHO requirements. The nucleotide sequence of VP1 region was determined, and the serotypes of NPEV were identified by molecular typing. The results showed that among the 82 strains of NPEV isolated from the AFP cases during 2011-2012, 42 isolates (55.3%) were identified as human enterovirus A (HEV-A), which were classified into 4 serotypes, 34 (44.7%) as human enterovirus B (HEV-B), which were classified into 13 serotypes, 2 as adenovirus, and 4 were untyped; human enteroviruses C and D were not found in these cases. Enterovirus A71 (EV-A71) was the main type of HEV-A, accounting for 85.7% of all HEV-A strains. HEV-A, especially EV-A71, was predominant among the NPEV strains isolated from AFP patients during 2011-2012 in Hebei Province.
本研究旨在调查2011 - 2012年中国河北省急性弛缓性麻痹（AFP）患者中分离出的非脊髓灰质炎肠道病毒（NPEV）的血清型分布，并分析这些病毒与AFP之间的关系。按照世界卫生组织的要求，使用人横纹肌肉瘤细胞（RD）和表达脊髓灰质炎病毒人细胞受体基因的小鼠细胞系（L20B），从河北省AFP病例的粪便标本中分离NPEV毒株。测定VP1区域的核苷酸序列，并通过分子分型鉴定NPEV的血清型。结果显示，在2011 - 2012年从AFP病例中分离出的82株NPEV中，42株（55.3%）被鉴定为人肠道病毒A（HEV - A），分为4个血清型；34株（44.7%）为人肠道病毒B（HEV - B），分为13个血清型；2株为腺病毒，4株未分型；未发现人肠道病毒C和D。肠道病毒A71（EV - A71）是HEV - A的主要类型，占所有HEV - A毒株的85.7%。在2011 - 2012年河北省AFP患者分离出的NPEV毒株中，HEV - A，尤其是EV - A71占主导地位。
