Haraguchi Kazutoshi, Kubota Kazuomi, Takada Tetsuo, Mahara Saori
Material Chemistry Laboratory, Kawamura Institute of Chemical Research, 631 Sakado, Sakura, Chiba, 285-0078, Japan.
Biomacromolecules. 2014 Jun 9;15(6):1992-2003. doi: 10.1021/bm401914c. Epub 2014 May 16.
Novel amphiphilic block copolymers composed of hydrophobic (poly(2-methoxyethyl acrylate): M) and hydrophilic (poly(N,N-dimethylacrylamide): D) segments were synthesized by living radical polymerization: a reversible addition-fragmentation chain-transfer polymerization. Two types of amphiphilic block copolymers, triblock (MDM) and 4-arm block ((MD)4) copolymers with specific compositions (D/M = (750-1500)/250), were prepared by a versatile one-pot synthesis. These copolymers show good adhesion to various types of substrates (e.g., polystyrene, polycarbonate, polypropylene, Ti, and glass), and the surface coating showed high protein repellency and a low contact angle for water, regardless of the substrate. The two opposing characteristics of high protein repellency and good substrate adhesion were achieved by the combined effects of the molecular architecture of the block copolymers, the high molecular weight, and the characteristics of each segment, that is, low protein adsorption capability of both segments and low glass transition temperature of the hydrophobic segment. Further, a polystyrene dish coated with the MDM block copolymer could be sterilized by γ-ray irradiation and used as a good substrate for a suspension cell culture that exhibits low cell adhesion and good cell growth.
通过活性自由基聚合（可逆加成-断裂链转移聚合）合成了由疏水链段（聚（丙烯酸2-甲氧基乙酯）：M）和亲水链段（聚（N，N-二甲基丙烯酰胺）：D）组成的新型两亲性嵌段共聚物。通过通用的一锅法合成制备了两种具有特定组成（D/M = (750 - 1500)/250）的两亲性嵌段共聚物，即三嵌段（MDM）和四臂嵌段（(MD)4）共聚物。这些共聚物对各种类型的基材（如聚苯乙烯、聚碳酸酯、聚丙烯、钛和玻璃）表现出良好的粘附性，并且无论基材如何，表面涂层都表现出高蛋白排斥性和低水接触角。通过嵌段共聚物的分子结构、高分子量以及每个链段的特性（即两个链段的低蛋白吸附能力和疏水链段的低玻璃化转变温度）的综合作用，实现了高蛋白排斥性和良好基材粘附性这两个相反的特性。此外，涂有MDM嵌段共聚物的聚苯乙烯培养皿可以通过γ射线辐照进行灭菌，并用作悬浮细胞培养的良好基材，该基材表现出低细胞粘附性和良好的细胞生长。
