Área de Toxicología, Facultad de Veterinaria, Universidad de Extremadura, Cáceres, Spain.
Altern Lab Anim. 2014 Mar;42(1):25-30. doi: 10.1177/026119291404200105.
Atrazine, prometryn, propazine and simazine are chlorotriazines that are commonly employed as herbicides. However, their use is a major cause of concern, due to their reported endocrine disrupting effects in different taxa. Data from studies on the molecular and cellular processes underlying the hormonal action of these substances are contradictory. The ability of these chlorotriazines and the atrazine metabolites, desethyl-s-chlorotriazine and desisopropyl-s-chlorotriazine, to trigger responses mediated by the oestrogen receptor (ER), aryl hydrocarbon receptor (AhR) and thyroid receptor (TR), was studied by using in vitro approaches. Transcriptional activation assays were applied to observe the activation of ER and TR. The induction of ethoxyresorufin-O-deethylase (EROD) activity in the RTG-2 cell line served as an indicator of AhR activation. No responses were found in any of the assays, with any of the six chlorotriazines tested. Our observations indicate that the chlorotriazines tested are unlikely to cause their endocrine effects via these receptors.
莠去津、扑灭津、扑灭净和西玛津是氯代三嗪类除草剂，被广泛应用。但由于它们被报道具有内分泌干扰作用，在不同分类群中引发关注。这些物质的激素作用的分子和细胞过程的研究数据存在矛盾。采用体外方法研究了这些氯代三嗪类物质以及莠去津代谢物去乙基-s-氯代三嗪和去异丙基-s-氯代三嗪，以探究它们是否能够触发雌激素受体 (ER)、芳烃受体 (AhR) 和甲状腺受体 (TR) 介导的反应。应用转录激活测定法观察 ER 和 TR 的激活。在 RTG-2 细胞系中诱导乙氧基荧光素-O-脱乙基酶 (EROD) 活性作为 AhR 激活的指标。在任何测定中，都没有观察到六种氯代三嗪类物质中的任何一种能够引发反应。我们的观察结果表明，所测试的氯代三嗪类物质不太可能通过这些受体引起其内分泌作用。
