Florian Colin P, Mansfield Shelly R, Schroeder Jennifer R
Department of Biology, Millikin University, Decatur, IL 62522, USA.
Biochem Res Int. 2016;2016:2984081. doi: 10.1155/2016/2984081. Epub 2016 Feb 3.
Over 200,000 cases of invasive breast cancer are diagnosed annually; herbicide contaminants in local water sources may contribute to the growth of these cancers. GPR30, a G protein coupled receptor, was identified as a potential orphan receptor that may interact with triazine herbicides such as atrazine, one of the most commonly utilized chlorotriazines in agricultural practices in the United States. Our goal was to identify whether chlorotriazines affected the expression of GPR30. Two breast cancer cell lines, MDA-MB-231 and MCF-7, as well as one normal breast cell line, MCF-10A, were treated with a 100-fold range of atrazine, cyanazine, or simazine, with levels flanking the EPA safe level for each compound. Using real-time PCR, we assessed changes in GPR30 mRNA compared to a GAPDH control. Our results indicate that GPR30 expression increased in breast cancer cells at levels lower than the US EPA drinking water contamination limit. During this treatment, the viability of cells was unaltered. In contrast, treatment with chlorotriazines reduced the expression of GPR30 in noncancerous MCF-10A cells. Thus, our results indicate that cell milieu and potential to metastasize may play a role in the extent of GPR30 response to pesticide exposure.
每年有超过20万例浸润性乳腺癌被确诊；当地水源中的除草剂污染物可能促使这些癌症的发展。GPR30是一种G蛋白偶联受体，被确定为一种潜在的孤儿受体，它可能与三嗪类除草剂相互作用，如阿特拉津，它是美国农业实践中最常用的氯三嗪之一。我们的目标是确定氯三嗪是否会影响GPR30的表达。用100倍浓度范围的阿特拉津、氰草津或西玛津处理两种乳腺癌细胞系MDA-MB-231和MCF-7以及一种正常乳腺细胞系MCF-10A，其浓度处于每种化合物的美国环境保护局安全水平两侧。使用实时聚合酶链反应，我们评估了与甘油醛-3-磷酸脱氢酶对照相比GPR30信使核糖核酸的变化。我们的结果表明，在低于美国环境保护局饮用水污染限值的水平下，乳腺癌细胞中GPR30的表达增加。在这种处理过程中，细胞的活力未改变。相比之下，用氯三嗪处理会降低非癌性MCF-10A细胞中GPR30的表达。因此，我们的结果表明，细胞环境和转移潜能可能在GPR30对农药暴露的反应程度中起作用。
