Bogoch Isaac I, Coulibaly Jean T, Andrews Jason R, Speich Benjamin, Keiser Jennifer, Stothard J Russell, N'goran Eliézer K, Utzinger Jürg
Divisions of Internal Medicine and Infectious Diseases,Toronto General Hospital,14EN-209, 200 Elizabeth Street, Toronto, ON, M5G 2C4,Canada.
Unité de Formation et de Recherche Biosciences,Université Félix Houphouët-Boigny,22 BP 770, Abidjan 22,Côte d'Ivoire.
Parasitology. 2014 Dec;141(14):1811-8. doi: 10.1017/S0031182014000432. Epub 2014 Apr 28.
The diagnosis of parasitic worm (helminth) infections requires specialized laboratory settings, but most affected individuals reside in locations without access to such facilities. We tested two portable microscopic devices for the diagnosis of helminth infections in a cross-sectional survey in rural Côte d'Ivoire. We examined 164 stool samples under a light microscope and then re-examined with a commercial portable light microscope and an experimental mobile phone microscope for the diagnosis of Schistosoma mansoni and soil-transmitted helminths. Additionally, 180 filtered urine samples were examined by standard microscopy and compared with the portable light microscope for detection of Schistosoma haematobium eggs. Conventional microscopy was considered the diagnostic reference standard. For S. mansoni, S. haematobium and Trichuris trichiura, the portable light microscope showed sensitivities of 84.8%, 78.6% and 81.5%, respectively, and specificities of 85.7%, 91.0% and 93.0%, respectively. For S. mansoni and T. trichiura, we found sensitivities for the mobile phone microscope of 68.2% and 30.8%, respectively, and specificities of 64.3% and 71.0%, respectively. We conclude that the portable light microscope has sufficient diagnostic yield for Schistosoma and T. trichiura infections, while the mobile phone microscope has only modest sensitivity in its current experimental set-up. Development of portable diagnostic technologies that can be used at point-of-sample collection will enhance diagnostic coverage in clinical and epidemiological settings.
寄生虫（蠕虫）感染的诊断需要专门的实验室环境，但大多数感染者居住在无法获得此类设施的地区。在科特迪瓦农村地区的一项横断面调查中，我们测试了两种便携式显微镜设备用于诊断蠕虫感染。我们在光学显微镜下检查了164份粪便样本，然后使用商用便携式光学显微镜和实验性手机显微镜重新检查，以诊断曼氏血吸虫和土壤传播的蠕虫。此外，通过标准显微镜检查了180份过滤后的尿液样本，并与便携式光学显微镜进行比较，以检测埃及血吸虫卵。传统显微镜检查被视为诊断参考标准。对于曼氏血吸虫、埃及血吸虫和鞭虫，便携式光学显微镜的敏感性分别为84.8%、78.6%和81.5%，特异性分别为85.7%、91.0%和93.0%。对于曼氏血吸虫和鞭虫，我们发现手机显微镜的敏感性分别为68.2%和30.8%，特异性分别为64.3%和71.0%。我们得出结论，便携式光学显微镜对血吸虫和鞭虫感染具有足够的诊断率，而手机显微镜在其当前实验设置中敏感性仅为中等。开发可在样本采集点使用的便携式诊断技术将提高临床和流行病学环境中的诊断覆盖率。
