Fischer H P, Wallner F, Maier H, Weber K, Osborn M, Altmannsberger M
Department of Pathology, University of Giessen, Max Planck Institute for Biophysical Chemistry, Federal Republic of Germany.
Lab Invest. 1989 Oct;61(4):433-9.
Frozen sections of 48 squamous cell carcinomas and seven undifferentiated carcinomas of the upper aerodigestive tract were investigated immunohistochemically with monoclonal antibodies specific for keratin, vimentin, desmin, neurofilaments, and glial fibrillary acidic proteins. In nine squamous cell carcinomas (19%) and six undifferentiated carcinomas (86%) obtained before treatment coexpression of keratin and vimentin was detected in some tumor cells by double immunofluorescence studies. Nine squamous cell carcinomas expressed neurofilaments in scattered tumor cells. Coexpression of vimentin or neurofilaments was seen especially in the peripheral cell layer of the tumor nests and did not seem to correlate with the degree of differentiation. Three undifferentiated carcinomas additionally expressed desmin, and one tumor contained neurofilaments. Glial fibrillary acidic proteins were not detected. Increased coexpression of keratin with vimentin, desmin, or neurofilaments was seen in some tumors that were studied before and after radiation/chemotherapy, suggesting that the intermediate filament profile of tumor cells can be altered by external influences.
采用针对角蛋白、波形蛋白、结蛋白、神经丝和胶质纤维酸性蛋白的单克隆抗体，对48例上消化道鳞状细胞癌和7例未分化癌的冰冻切片进行免疫组织化学研究。在治疗前获取的9例鳞状细胞癌（19%）和6例未分化癌（86%）中，通过双重免疫荧光研究在一些肿瘤细胞中检测到角蛋白和波形蛋白的共表达。9例鳞状细胞癌在散在的肿瘤细胞中表达神经丝。波形蛋白或神经丝的共表达尤其见于肿瘤巢的外周细胞层，似乎与分化程度无关。3例未分化癌还表达结蛋白，1例肿瘤含有神经丝。未检测到胶质纤维酸性蛋白。在一些放疗/化疗前后研究的肿瘤中，可见角蛋白与波形蛋白、结蛋白或神经丝的共表达增加，提示肿瘤细胞的中间丝谱可受外部影响而改变。
