Meléndez-Martínez Antonio J, Stinco Carla M, Brahm Paula Mapelli, Vicario Isabel M
Facultad de Farmacia, Department of Nutrition & Food Science, Food Colour & Quality Laboratory, Universidad de Sevilla, P. García González 2, 41012, Sevilla, Spain,
Methods Mol Biol. 2014;1153:77-97. doi: 10.1007/978-1-4939-0606-2_6.
Carotenoids and tocopherols are lipid secondary metabolites that play essential roles in plants. They are also relevant compounds from a nutritional standpoint and attract much attention due to their proposed antioxidant properties. In this chapter, methodologies for the extraction and HPLC analysis of these compounds are described as well as a widely used protocol to assess their antioxidant capacity.
类胡萝卜素和生育酚是植物中起重要作用的脂质次生代谢产物。从营养角度来看,它们也是相关化合物,因其具有抗氧化特性而备受关注。在本章中,将描述这些化合物的提取方法和高效液相色谱分析方法,以及一种广泛用于评估其抗氧化能力的方案。