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植物排放生物源挥发性有机化合物的气相色谱-质谱测定法。

Gas chromatography-mass spectrometry method for determination of biogenic volatile organic compounds emitted by plants.

作者信息

Kännaste Astrid, Copolovici Lucian, Niinemets Ülo

机构信息

Institute of Agricultural and Environmental Sciences, Estonian University of Life Sciences, Kreutzwaldi 1, Tartu, 51014, Estonia.

出版信息

Methods Mol Biol. 2014;1153:161-9. doi: 10.1007/978-1-4939-0606-2_11.

Abstract

Gas chromatography-mass spectrometry (GC-MS) is one of the most widely used methods for analyzing the emissions of biogenic volatile organic compounds (VOCs) from plants. Preconcentration of VOCs on the cartridges filled with different adsorbents is a well-accepted method for sampling of headspace. Here, we describe a gas-chromatographic method for determination of different isoprenoids (isoprene, monoterpenes, homoterpenes, and sesquiterpenes). The technique is based on adsorption of compounds of interest on multibed adsorbent cartridges followed by thermodesorption, and detection and analysis by GC-MS.

摘要

气相色谱-质谱联用(GC-MS)是分析植物生物源挥发性有机化合物(VOCs)排放最广泛使用的方法之一。在填充不同吸附剂的小柱上预浓缩VOCs是一种广泛认可的顶空采样方法。在此,我们描述一种用于测定不同类异戊二烯(异戊二烯、单萜、高萜和倍半萜)的气相色谱方法。该技术基于将目标化合物吸附在多层吸附剂小柱上,然后进行热脱附,并通过GC-MS进行检测和分析。

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