Eshkoor Sa, Ismail P, Rahman Sa, Moin S, Adon My
Institute of Gerontology, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia ; Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia.
Institute of Gerontology, Universiti Putra Malaysia (UPM), Serdang, Selangor, Malaysia.
Balkan J Med Genet. 2013 Dec;16(2):45-52. doi: 10.2478/bjmg-2013-0031.
The ageing process is influenced by many internal and external factors. The toxic substances in the environment can cause genomic damages to cells, which increase the risk of early ageing. Furthermore, the cytochrome P450 1A2 (CYP1A2) gene polymorphism is a susceptibility factor and may enhance the risk of DNA damage in cells. The current study was carried out to show whether occupational exposure could cause genotoxicity in cells carrying the CYP1A2 gene polymorphism, thus enhancing the likelihood of early ageing. This study was conducted on mechanical workshop workers and a control group by collecting buccal cells from their mouths. Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) was used to identify the CYP1A2 gene polymorphism in the cells. In addition, three extra methods including micronuclei (MN) test, comet assay and real-time PCR (RT-PCR) were applied to determine the effects of gene polymorphisms on DNA damage and ageing from occupational exposure. The results showed that DNA damage in the cells carrying the mutated genotype was higher than the wild genotype. In addition, the difference in MN frequency (p = 0.001) and relative telomere length (p = 0.002) between workers and controls was significant (p <0.05) in the mutated genotype. The findings indicated a possible protective effect of gene polymorphism against early ageing, which was characterized by lack of a significant influence of CYP1A2 gene polymorphism on genetic material in the subjects (p >0.05). It was concluded that the CYP1A2 gene could be a contributing factor to prevent early ageing from occupational exposure.
衰老过程受到许多内部和外部因素的影响。环境中的有毒物质可导致细胞基因组损伤，增加早衰风险。此外，细胞色素P450 1A2（CYP1A2）基因多态性是一个易感因素，可能会增加细胞中DNA损伤的风险。本研究旨在表明职业暴露是否会对携带CYP1A2基因多态性的细胞造成遗传毒性，从而增加早衰的可能性。本研究以机械车间工人及其对照组为研究对象，采集他们口腔中的颊细胞。采用限制性片段长度多态性-聚合酶链反应（RFLP-PCR）鉴定细胞中的CYP1A2基因多态性。此外，还应用了微核（MN）试验、彗星试验和实时荧光定量PCR（RT-PCR）这三种额外的方法来确定基因多态性对职业暴露导致的DNA损伤和衰老的影响。结果显示，携带突变基因型的细胞中的DNA损伤高于野生基因型。此外，在突变基因型中，工人与对照组之间的MN频率差异（p = 0.001）和相对端粒长度差异（p = 0.002）具有显著性（p <0.05）。研究结果表明基因多态性可能对早衰具有保护作用，其特征是CYP1A2基因多态性对受试者遗传物质没有显著影响（p >0.05）。研究得出结论，CYP1A2基因可能是预防职业暴露导致早衰的一个因素。
