Bidar Maryam, Zarrabi Mohammad Hasan, Tavakol Afshari Jalil, Aghasizadeh Navid, Naghavi Neda, Forghanirad Maryam, Attaran Niloufar
Department of Endodontics, Dental Research Center/Mashad University of Medical Sciences, Mashad, Iran.
Department of Immunology, Dental School, Mashad University of Medical Sciences, Mashad, Iran.
Iran Endod J. 2011 Summer;6(3):111-5. Epub 2011 Aug 15.
The materials used for root-end filling and perforation repair are in direct contact with live tissues e.g. bone and connective tissue; their effects however, are uncertain. The aim of this ex vivostudy was to evaluate the osteoblastic secretory activity adjacent to gray and white mineral trioxide aggregate (MTA) and Intermediate Restorative Material (IRM).
The studied materials were prepared and placed in 24-wells plate. Human MG-63 osteoblasts were introduced to materials after their initial set. The supernatant fluid was collected after 1, 3, and 7 days and the level of interleukin-1β was measured by ELISA test. A microscopic exam was also performed to assess proliferation and viability of the cells. Kruskal-Wallis and Tukey tests were used for analysis.
T here were significant higher levels of interleukin-1β in the gray and white MTA groups compared to IRM group (P<0.05). The difference in interleukin-1β secretion level between two mineral trioxide aggregate groups was not significant (P>0.05).Morphologic appearance of osteoblasts adjacent to gray and white MTA was similar to normal osteoblasts in all observation periods, however cells adjacent to IRM were round, signifying cytotoxicity of the adjacent material.
Human osteoblasts' has a favorable biologic response to white and gray MTA compared to IRM.
用于根尖充填和穿孔修复的材料与活组织如骨和结缔组织直接接触；然而，它们的作用尚不确定。本体外研究的目的是评估灰色和白色矿物三氧化物凝聚体（MTA）及中间修复材料（IRM）邻近区域的成骨细胞分泌活性。
制备研究材料并置于24孔板中。在材料初步凝固后将人MG-63成骨细胞接种于材料上。分别在1、3和7天后收集上清液，通过酶联免疫吸附测定（ELISA）检测白细胞介素-1β水平。还进行了显微镜检查以评估细胞的增殖和活力。采用Kruskal-Wallis和Tukey检验进行分析。
与IRM组相比，灰色和白色MTA组的白细胞介素-1β水平显著更高（P<0.05）。两种矿物三氧化物凝聚体组之间白细胞介素-1β分泌水平的差异不显著（P>0.05）。在所有观察期内，与灰色和白色MTA相邻的成骨细胞形态与正常成骨细胞相似，然而与IRM相邻的细胞呈圆形，表明相邻材料具有细胞毒性。
与IRM相比，人成骨细胞对白色和灰色MTA具有良好的生物学反应。
