Koh E T, McDonald F, Pitt Ford T R, Torabinejad M
Department of Conservative Dentistry, United Medical School, London, UK.
J Endod. 1998 Aug;24(8):543-7. doi: 10.1016/S0099-2399(98)80074-5.
This investigation studied the cytomorphology of osteoblasts in the presence of Mineral Trioxide Aggregate (MTA) and examined cytokine production. MTA and Intermediate Restorative Material (IRM) were prepared and placed in separate Petri dishes. Osteoblasts (cell-line MG-63), grown to confluence in Hams F12/Dulbecco's modified Eagle's medium, were seeded into the dishes, which were incubated for 1 to 7 days. The specimens were viewed by scanning electron microscopy. For cytokine evaluation, cells were grown either alone or in other dishes containing the test materials for 1 to 144 h. Media were removed for ELISA analysis of interleukin (IL)-1 alpha, IL-1 beta, IL-6, and macrophage colony-stimulating factor. Scanning electron microscopy revealed healthy cells in contact with MTA at 1 and 3 days; in contrast, cells in the presence of IRM appeared rounded. The ELISA assays revealed raised levels of all ILs at all periods when cells were grown in the presence of MTA; in contrast, cells grown alone or with IRM produced undetectable amounts. The macrophage colony-stimulating factor was produced by cells irrespective of the group. It seems that MTA offers a biologically active substrate for bone cells and stimulates IL production.
本研究探讨了在三氧化矿物凝聚体（MTA）存在的情况下成骨细胞的细胞形态，并检测了细胞因子的产生。制备了MTA和中间修复材料（IRM），并将它们分别置于培养皿中。将在汉氏F12/杜尔贝科改良伊格尔培养基中生长至汇合的成骨细胞（MG-63细胞系）接种到培养皿中，培养1至7天。通过扫描电子显微镜观察标本。为了评估细胞因子，细胞单独培养或在含有测试材料的其他培养皿中培养1至144小时。收集培养基用于酶联免疫吸附测定（ELISA）分析白细胞介素（IL）-1α、IL-1β、IL-6和巨噬细胞集落刺激因子。扫描电子显微镜显示，在第1天和第3天时，与MTA接触的细胞健康；相比之下，存在IRM时的细胞呈圆形。ELISA分析显示，当细胞在MTA存在的情况下生长时，所有时期的所有白细胞介素水平均升高；相比之下，单独培养或与IRM一起培养的细胞产生的量无法检测到。无论在哪一组中，细胞均产生巨噬细胞集落刺激因子。似乎MTA为骨细胞提供了一种生物活性底物，并刺激白细胞介素的产生。
