Yan Shu-xiang, Xiong Mei-hua, Zhu Bin, Tian Hua, Liao Xiao-lin, Shao Ke
a Key Laboratory of Ecological Impacts of Hydraulic-Projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources , Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences , Wuhan , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):698-9. doi: 10.3109/19401736.2014.913149. Epub 2014 Apr 29.
In the present study, the complete mitochondrial genome sequence of Rhinogobio typus (Teleostei, Cyprinidae, Gobioninae) was obtained using a PCR-based method. The total length of the mitochondrial genome is 16,608 bp, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and a non-coding control region (D-loop), which is similar to that observed in many other reported fishes. The overall-based composition is 31.3% A, 26.6% T, 15.9% G and 26.2% C, with high A + T content (57.9%). These results will provide a useful tool for species identification, evolutionary and population genetic studies of R. typus and its related species.
在本研究中,采用基于PCR的方法获得了吻鮈(硬骨鱼纲,鲤科,鮈亚科)的完整线粒体基因组序列。线粒体基因组全长16,608 bp,包括22个转运RNA基因、2个核糖体RNA基因、13个蛋白质编码基因和一个非编码控制区(D环),这与许多其他已报道鱼类中观察到的情况相似。碱基总体组成为31.3%A、26.6%T、15.9%G和26.2%C,A+T含量较高(57.9%)。这些结果将为吻鮈及其相关物种的物种鉴定、进化和群体遗传学研究提供有用的工具。