Chen I-Shiung, Liao Chiao-Ru, Shao Kwang-Tsao
a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan , ROC and.
b Biodiversity Research Center, Academia Sinica , Taipei , Taiwan , ROC.
Mitochondrial DNA. 2015;26(6):829-31. doi: 10.3109/19401736.2013.855914. Epub 2014 Jan 10.
In this study, the complete mitogenome sequence of cyprinid fish, Taiwanese barbel steed, Hemibarbus schlegelii (Günther, 1868) (Teleostei: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,612 bp, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a non-coding control region (CR). CR of 933 bp length is located between tRNAPro and tRNAPhe. The overall base composition of Hemibarbus schlegelii is 29.9% for A, 27.2% for C, 25.9% for T and 17.0% for G, with a slight AT bias of 55.8%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.
在本研究中，采用长聚合酶链反应方法扩增并测序了鲤科鱼类台湾长吻鮠（Hemibarbus schlegelii (Günther, 1868)）（硬骨鱼纲：鲤科）的完整线粒体基因组序列。该线粒体基因组由16,612个碱基对组成，具有典型的脊椎动物线粒体基因排列，包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个非编码控制区（CR）。长度为933个碱基对的CR位于tRNAPro和tRNAPhe之间。台湾长吻鮠的总体碱基组成为：A占29.9%，C占27.2%，T占25.9%，G占17.0%，AT偏倚率为55.8%。完整的线粒体基因组可能为不仅是同属物种，而且是鲤科鱼类更高分类阶元的进一步系统发育分析提供相当重要的DNA分子数据。
