Chen I-Shiung, Liu Yu-Wei, Huang Shih-Pin, Shen Chia-Ning
a Institute of Marine Biology and.
b Institute of Aquaculture, National Taiwan Ocean University , Keelung , Taiwan , ROC , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):708-10. doi: 10.3109/19401736.2014.913153. Epub 2014 Apr 29.
The complete mitochondrial genome of the Korean minnow Nipponocypris koreanus (Kim et al., 2005) which is endemic to Korean Peninsula has been amplified and sequenced in this study. The complete mitochondrial genome is 16,615 base pairs (bp) in total length, had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 2 rRNAs, 22 tRNAs, 1 control region (CR) and 1 light-strand replication origin (OL). The overall base composition of N. koreanus is 30.0% for A, 26.8% for T, 26.1% for C, 17.1% for G, with higher AT content 56.8%. This study will be rather beneficial for understanding the phylogenetic approach of Nipponocypris species and other related genera of cyprinid fishes.
本研究对韩国半岛特有的韩国小鲤(Nipponocypris koreanus,Kim等人,2005)的完整线粒体基因组进行了扩增和测序。完整的线粒体基因组全长16,615个碱基对(bp),具有典型的脊椎动物线粒体基因排列,包括13个蛋白质编码基因、2个rRNA、22个tRNA、1个控制区(CR)和1个轻链复制起点(OL)。韩国小鲤的总体碱基组成为:A占30.0%,T占26.8%,C占26.1%,G占17.1%,AT含量较高,为56.8%。本研究将有助于了解韩国小鲤属及鲤科鱼类其他相关属的系统发育关系。