Que Shengquan, Zou Zhiwen, Xin Tianrong, Xia Bin
a School of Life Sciences and Food Engineering, Nanchang University , Nanchang 330031 , China and.
b Center for Watershed Ecology, Institute of Life Science, Nanchang University , Nanchang 330031 , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):688-9. doi: 10.3109/19401736.2014.913144. Epub 2014 Apr 29.
In this study, the first complete mitochondrial genome (mitogenome) of the mold mite, Tyrophagus putrescentiae was determined by long PCR and primer walking methods. The mitogenome was 13,288 bp in length, including 13 typical invertebrate protein-coding genes, 18 transfer RNA genes and 2 ribosomal RNA genes. The overall base composition of the heavy strand is 22.5% A, 48.2% T, 13.2% C and 16.1% G, with a A+T bias. Stop codon was incomplete for ND4 gene and ND2. Gene overlaps were observed between 12 pairs of the contiguous genes in T. putrescentiae. The study also displayed that four tRNAs were missed for T. putrescentiae. Researches on mitogenome would further contribute to resolving phylogenetic position and interrelationships of Tyrophagus.
在本研究中，通过长PCR和引物步移法测定了腐食酪螨（Tyrophagus putrescentiae）的首个完整线粒体基因组（线粒体基因组）。该线粒体基因组长度为13288 bp，包括13个典型的无脊椎动物蛋白质编码基因、18个转移RNA基因和2个核糖体RNA基因。重链的总体碱基组成为22.5%A、48.2%T、13.2%C和16.1%G，具有A+T偏向性。ND4基因和ND2的终止密码子不完整。在腐食酪螨的12对相邻基因之间观察到基因重叠。该研究还表明，腐食酪螨缺少4个tRNA。对线粒体基因组的研究将进一步有助于解决腐食酪螨的系统发育位置和相互关系。
